Literature DB >> 1793014

Phospholipase A2 (PLA2) activity in bovine pulmonary artery endothelial cells.

R Goodman1, T M Stevens, L R Mantegna, P R Kidd, R R Harris, J S Kerr.   

Abstract

We have investigated the role of recombinant human interleukin-1 beta (rIL-1 beta) and recombinant human tumor necrosis factor alpha (rTNF-alpha) on PLA2 activity, protein synthesis and eicosanoid production in bovine pulmonary artery endothelial cells. Cellular PLA2 activity increased 4-fold and production of PGE2 increased 3-fold at 1-2 hrs in the presence of 10 units/ml rIL-1 beta. PLA2 activity increased 3-fold at 30 min and PGE2 production increased 2-fold with 5 x 10(-9) M rTNF-alpha. The data show that endothelial cells respond more rapidly to rIL-1 beta (2-6 hr) and rTNF-alpha (30 min) than do chondrocytes and synovial cells (6-16 hrs), suggesting endothelial cells may play a primary role in initiating the inflammatory response.

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Year:  1991        PMID： 1793014     DOI： 10.1007/bf01993253

Source DB:  PubMed          Journal:  Agents Actions        ISSN： 0065-4299


  8 in total

1.  Phospholipases and phospholipid turnover in Escherichia coli spheroplasts.

Authors:  P Patriarca; S Beckerdite; P Elsbach
Journal:  Biochim Biophys Acta       Date:  1972-04-18

2.  Effects of recombinant interleukin-1 beta on phospholipase A2 activity, phospholipase A2 mRNA levels, and eicosanoid formation in rabbit chondrocytes.

Authors:  J S Kerr; T M Stevens; G L Davis; J A McLaughlin; R R Harris
Journal:  Biochem Biophys Res Commun       Date:  1989-12-29       Impact factor: 3.575

Review 3.  Role of endothelium in chronic inflammation.

Authors:  M Ziff
Journal:  Springer Semin Immunopathol       Date:  1989

4.  Tumor necrosis factors alpha and beta differ in their capacities to generate interleukin 1 release from human endothelial cells.

Authors:  R M Locksley; F P Heinzel; H M Shepard; J Agosti; T E Eessalu; B B Aggarwal; J M Harlan
Journal:  J Immunol       Date:  1987-09-15       Impact factor: 5.422

5.  Interleukin 1 activates phospholipase A2 in rabbit chondrocytes: a possible signal for IL 1 action.

Authors:  J Chang; S C Gilman; A J Lewis
Journal:  J Immunol       Date:  1986-02-15       Impact factor: 5.422

6.  Tumour necrosis factor (cachectin) induces phospholipase A2 activity and synthesis of a phospholipase A2-activating protein in endothelial cells.

Authors:  M A Clark; M J Chen; S T Crooke; J S Bomalaski
Journal:  Biochem J       Date:  1988-02-15       Impact factor: 3.857

7.  Phospholipase A2 activation by interleukin 1: release and metabolism of arachidonic acid by IL 1-stimulated rabbit chondrocytes.

Authors:  S C Gilman; P R Berner; J Chang
Journal:  Agents Actions       Date:  1987-08

8.  Inhibition of phospholipase A2 by "lipocortins" and calpactins. An effect of binding to substrate phospholipids.

Authors:  F F Davidson; E A Dennis; M Powell; J R Glenney
Journal:  J Biol Chem       Date:  1987-02-05       Impact factor: 5.157
  8 in total
  1 in total

1.  IL-1 and its role in rat carrageenan pleurisy.

Authors:  R Goodman; L R Mantegna; C L McAilister; E Bruin; R L Dowling; H George; W Feeser; B Freimark; M Lischwe; S Pick; R R Harris; J S Kerr
Journal:  Mediators Inflamm       Date:  1993       Impact factor: 4.711
  1 in total

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