SUMMARY OBJECTIVE: Characterize mycobacterial species causing pulmonary tuberculosis (PTB) at the Korle-Bu Teaching Hospital in Ghana. DESIGN: Sputum smear positive samples, two (2) from 70 patients diagnosed as having tuberculosis, after they had consented, were collected from the Korle-Bu Teaching Hospital Chest Clinic between January and July 2003. SETTING: Korle-Bu Teaching Hospital Chest Clinic, Accra. RESULTS: Sixty-four mycobacterial isolates were obtained and confirmed as members of Mycobacterium tuberculosis complex by colonial morphology and conventional biochemical assays. Forty-seven (73%) were M. tuberculosis, the human strain, 2 (3%) M. bovis, the bovine strain, 13 (20%) M. africanum I (West Africa type), and 2 (3%) M. africanum II (East Africa type). CONCLUSION: The results indicate that, there are various strains causing PTB at the Korle-Bu Teaching Hospital and of great concern is M. bovis, which mostly causes extra-PTB in humans but found to cause PTB in this study. This calls for the need to conduct a nationwide survey using both conventional and molecular techniques to characterize various mycobacterial species causing TB in Ghana. This will result in better understanding of the various strains circulating in the country and inform individual TB treatment regimen especially the inclusion or exclusion of pyrazinamide.
SUMMARY OBJECTIVE: Characterize mycobacterial species causing pulmonary tuberculosis (PTB) at the Korle-Bu Teaching Hospital in Ghana. DESIGN: Sputum smear positive samples, two (2) from 70 patients diagnosed as having tuberculosis, after they had consented, were collected from the Korle-Bu Teaching Hospital Chest Clinic between January and July 2003. SETTING: Korle-Bu Teaching Hospital Chest Clinic, Accra. RESULTS: Sixty-four mycobacterial isolates were obtained and confirmed as members of Mycobacterium tuberculosis complex by colonial morphology and conventional biochemical assays. Forty-seven (73%) were M. tuberculosis, the human strain, 2 (3%) M. bovis, the bovine strain, 13 (20%) M. africanum I (West Africa type), and 2 (3%) M. africanum II (East Africa type). CONCLUSION: The results indicate that, there are various strains causing PTB at the Korle-Bu Teaching Hospital and of great concern is M. bovis, which mostly causes extra-PTB in humans but found to cause PTB in this study. This calls for the need to conduct a nationwide survey using both conventional and molecular techniques to characterize various mycobacterial species causing TB in Ghana. This will result in better understanding of the various strains circulating in the country and inform individual TB treatment regimen especially the inclusion or exclusion of pyrazinamide.
Entities:
Keywords:
Bovine Tuberculosis; M. africanum; M. bovis; M. tuberculosis; Pulmonary Tuberculosis
Authors: M Dosso; D Bonard; P Msellati; A Bamba; C Doulhourou; V Vincent; M Peyre; M Traore; K Koffi; I M Coulibaly Journal: Int J Tuberc Lung Dis Date: 1999-09 Impact factor: 2.373
Authors: Linda M Parsons; Roland Brosch; Stewart T Cole; Akos Somoskövi; Arthur Loder; Gisela Bretzel; Dick Van Soolingen; Yvonne M Hale; Max Salfinger Journal: J Clin Microbiol Date: 2002-07 Impact factor: 5.948
Authors: Debby V Cousins; Ricardo Bastida; Angel Cataldi; Viviana Quse; Sharon Redrobe; Sue Dow; Padraig Duignan; Alan Murray; Christine Dupont; Niyaz Ahmed; Des M Collins; W Ray Butler; David Dawson; Diego Rodríguez; Julio Loureiro; Maria Isabel Romano; A Alito; M Zumarraga; Amelia Bernardelli Journal: Int J Syst Evol Microbiol Date: 2003-09 Impact factor: 2.747
Authors: Balako Gumi; Esther Schelling; Rebuma Firdessa; Abraham Aseffa; Rea Tschopp; Lawrence Yamuah; Douglas Young; Jakob Zinsstag Journal: Trop Anim Health Prod Date: 2011-04-18 Impact factor: 1.559