| Literature DB >> 17922018 |
Marlene Oeffinger1, Karen E Wei, Richard Rogers, Jeffrey A DeGrasse, Brian T Chait, John D Aitchison, Michael P Rout.
Abstract
The study of the dynamic interactome of cellular ribonucleoprotein (RNP) particles has been hampered by severe methodological limitations. In particular, the affinity purification of intact RNP complexes from cell lysates suffers from RNA degradation, loss of interacting macromolecules and poor overall yields. Here we describe a rapid affinity-purification method for efficient isolation of the subcomplexes that dynamically organize different RNP biogenesis pathways in Saccharomyces cerevisiae. Our method overcomes many of the previous limitations to produce large RNP interactomes with almost no contamination.Entities:
Mesh：
Substances：
Year: 2007 PMID： 17922018 DOI： 10.1038/nmeth1101
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547