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Literature DB >> 17913625

The use of fungal in vitro systems for studying translational regulation.

Cheng Wu¹, Nadia Amrani, Allan Jacobson, Matthew S Sachs.

Abstract

The use of cell-free systems enables biochemical determination of factors and mechanisms contributing to translational processes. The preparation and use of cell-free translation systems from the fungi Saccharomyces cerevisiae and Neurospora crassa are described. Examples provided illustrate the use of these systems, in conjunction with luciferase assays, [(35)S]Met incorporation, and primer-extension inhibition (toeprint) analyses, to assess the translational effects of upstream open reading frames and premature termination codons.

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Year: 2007 PMID： 17913625 DOI： 10.1016/S0076-6879(07)29010-X

Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600

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Cited

26 in total

The use of fungal in vitro systems for studying translational regulation.

1. Tail-anchor targeting by a Get3 tetramer: the structure of an archaeal homologue.

2. Translation factors promote the formation of two states of the closed-loop mRNP.

3. Stm1 modulates translation after 80S formation in Saccharomyces cerevisiae.

4. Gle1 Regulates RNA Binding of the DEAD-Box Helicase Ded1 in Its Complex Role in Translation Initiation.

5. Translational competence of ribosomes released from a premature termination codon is modulated by NMD factors.

6. Arginine changes the conformation of the arginine attenuator peptide relative to the ribosome tunnel.

7. The DEAD-box protein Ded1 modulates translation by the formation and resolution of an eIF4F-mRNA complex.

8. Codon usage regulates human KRAS expression at both transcriptional and translational levels.

9. Precise timing of ATPase activation drives targeting of tail-anchored proteins.

10. Codon Usage Influences the Local Rate of Translation Elongation to Regulate Co-translational Protein Folding.