Literature DB >> 17906633

An activity-independent selection system of thermostable protein variants.

Hélène Chautard1, Emilio Blas-Galindo, Thierry Menguy, Laure Grand'Moursel, Felipe Cava, José Berenguer, Marc Delcourt.   

Abstract

We describe an activity-independent method for the selection of thermostable mutants of any protein. It is based on a fusion construct comprising the protein of interest and a thermostable antibiotic resistance reporter, in such a way that thermostable mutants provide increased resistance in a thermophile. We isolated thermostable mutants of three human interferons and of two enzymes to demonstrate the applicability of the system.

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Year:  2007        PMID： 17906633     DOI： 10.1038/nmeth1090

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  7 in total

1.  A rapid protein folding assay for the bacterial periplasm.

Authors:  Thomas J Mansell; Stephen W Linderman; Adam C Fisher; Matthew P DeLisa
Journal:  Protein Sci       Date:  2010-05       Impact factor: 6.725

2.  Transcriptome and growth efficiency comparisons of recombinant thermophiles that produce thermolabile and thermostable proteins: implications for burden-based selection of thermostable proteins.

Authors:  Hirokazu Suzuki; Yuta Okumura; Yui Mikawa; Mao Takata; Shunsuke Yoshimura; Takashi Ohshiro
Journal:  Extremophiles       Date:  2021-06-30       Impact factor: 2.395

3.  Thermoadaptation-directed enzyme evolution in an error-prone thermophile derived from Geobacillus kaustophilus HTA426.

Authors:  Hirokazu Suzuki; Jyumpei Kobayashi; Keisuke Wada; Megumi Furukawa; Katsumi Doi
Journal:  Appl Environ Microbiol       Date:  2014-10-17       Impact factor: 4.792

Review 4.  Thermus thermophilus as biological model.

Authors:  Felipe Cava; Aurelio Hidalgo; José Berenguer
Journal:  Extremophiles       Date:  2009-01-21       Impact factor: 2.395

5.  Are in vivo selections on the path to extinction?

Authors:  José Berenguer; Mario Mencía; Aurelio Hidalgo
Journal:  Microb Biotechnol       Date:  2017-01-03       Impact factor: 5.813

6.  High-efficiency transformation of archaea by direct PCR products with its application to directed evolution of a thermostable enzyme.

Authors:  Yunhong Song; Zhiguang Zhu; Wei Zhou; Yi-Heng P Job Zhang
Journal:  Microb Biotechnol       Date:  2020-06-29       Impact factor: 5.813

7.  Optimizing protein stability in vivo.

Authors:  Linda Foit; Gareth J Morgan; Maximilian J Kern; Lenz R Steimer; Annekathrin A von Hacht; James Titchmarsh; Stuart L Warriner; Sheena E Radford; James C A Bardwell
Journal:  Mol Cell       Date:  2009-12-11       Impact factor: 17.970
  7 in total

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