Literature DB >> 17905883

Evaluation of postharvest-processed oysters by using PCR-based most-probable-number enumeration of Vibrio vulnificus bacteria.

Anita C Wright1, Victor Garrido, Georgia Debuex, Melissa Farrell-Evans, Archana A Mudbidri, W Steven Otwell.   

Abstract

Postharvest processing (PHP) is used to reduce levels of Vibrio vulnificus in oysters, but process validation is labor-intensive and expensive. Therefore, quantitative PCR was evaluated as a rapid confirmation method for most-probable-number enumeration (QPCR-MPN) of V. vulnificus bacteria in PHP oysters. QPCR-MPN showed excellent correlation (R(2) = 0.97) with standard MPN and increased assay sensitivity and efficiency.

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Year:  2007        PMID： 17905883      PMCID： PMC2168190          DOI： 10.1128/AEM.01118-07

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  23 in total

1.  Product differentiation by analysis of DNA melting curves during the polymerase chain reaction.

Authors:  K M Ririe; R P Rasmussen; C T Wittwer
Journal:  Anal Biochem       Date:  1997-02-15       Impact factor: 3.365

2.  Clinical manifestations and molecular epidemiology of Vibrio vulnificus infections in Denmark.

Authors:  A Dalsgaard; N Frimodt-Møller; B Bruun; L Høi; J L Larsen
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1996-03       Impact factor: 3.267

3.  Influence of water temperature and salinity on Vibrio vulnificus in Northern Gulf and Atlantic Coast oysters (Crassostrea virginica).

Authors:  M L Motes; A DePaola; D W Cook; J E Veazey; J C Hunsucker; W E Garthright; R J Blodgett; S J Chirtel
Journal:  Appl Environ Microbiol       Date:  1998-04       Impact factor: 4.792

4.  Densities of Vibrio vulnificus in the intestines of fish from the U.S. Gulf Coast.

Authors:  A DePaola; G M Capers; D Alexander
Journal:  Appl Environ Microbiol       Date:  1994-03       Impact factor: 4.792

5.  Use of the polymerase chain reaction in detection of culturable and nonculturable Vibrio vulnificus cells.

Authors:  L A Brauns; M C Hudson; J D Oliver
Journal:  Appl Environ Microbiol       Date:  1991-09       Impact factor: 4.792

6.  Isolation and characterization of Vibrio vulnificus from two Florida estuaries.

Authors:  M Tamplin; G E Rodrick; N J Blake; T Cuba
Journal:  Appl Environ Microbiol       Date:  1982-12       Impact factor: 4.792

7.  Disease caused by a marine Vibrio. Clinical characteristics and epidemiology.

Authors:  P A Blake; M H Merson; R E Weaver; D G Hollis; P C Heublein
Journal:  N Engl J Med       Date:  1979-01-04       Impact factor: 91.245

8.  Effect of temperature and salinity on Vibrio (Beneckea) vulnificus occurrence in a Gulf Coast environment.

Authors:  M T Kelly
Journal:  Appl Environ Microbiol       Date:  1982-10       Impact factor: 4.792

9.  Distribution of Vibrio vulnificus and other lactose-fermenting vibrios in the marine environment.

Authors:  J D Oliver; R A Warner; D R Cleland
Journal:  Appl Environ Microbiol       Date:  1983-03       Impact factor: 4.792

10.  Distribution of Vibrio vulnificus in the Chesapeake Bay.

Authors:  A C Wright; R T Hill; J A Johnson; M C Roghman; R R Colwell; J G Morris
Journal:  Appl Environ Microbiol       Date:  1996-02       Impact factor: 4.792
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  3 in total

1.  Quantification of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae in French Mediterranean coastal lagoons.

Authors:  Franck Cantet; Dominique Hervio-Heath; Audrey Caro; Cécile Le Mennec; Caroline Monteil; Catherine Quéméré; Anne Jolivet-Gougeon; Rita R Colwell; Patrick Monfort
Journal:  Res Microbiol       Date:  2013-06-12       Impact factor: 3.992

2.  Vancomycin-Resistant Enterococci and Bacterial Community Structure following a Sewage Spill into an Aquatic Environment.

Authors:  Suzanne Young; Bina Nayak; Shan Sun; Brian D Badgley; Jason R Rohr; Valerie J Harwood
Journal:  Appl Environ Microbiol       Date:  2016-08-30       Impact factor: 4.792

Review 3.  Food Safety Impacts from Post-Harvest Processing Procedures of Molluscan Shellfish.

Authors:  George L Baker
Journal:  Foods       Date:  2016-04-18
  3 in total

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