BACKGROUND: This study was designed to develop a simple, reversible primate middle cerebral artery (MCA) occlusion (MCAO) model. METHODS: MCAO was achieved by inserting a specially designed intraluminal balloon catheter from the external carotid artery, then to the internal carotid artery, and finally into the proximal segment of MCA using 13 adolescent monkeys (Macaca mulatta) weighing 4.25 +/- 0.23 kg. The infarction was confirmed by magnetic resonance imaging at 3, 7, and 23 hours after MCAO. RESULTS: Local cerebral blood flow in the caudate nucleus decreased remarkably. Post-MCAO electroencephalographic monitoring showed the inhibition of electroencephalography with slow waves distinctly increasing. Neurologic evaluation demonstrated persistent deficits in the ischemic group. Neuropathologic examinations were consistent with the neurologic evaluations. CONCLUSION: The results suggested that this reversible monkey model resembled a human stroke and should offer a reasonable alternative to craniotomy and the extrinsic ligation of the MCA in the laboratory investigation of focal reversible cerebral ischemia.
BACKGROUND: This study was designed to develop a simple, reversible primate middle cerebral artery (MCA) occlusion (MCAO) model. METHODS:MCAO was achieved by inserting a specially designed intraluminal balloon catheter from the external carotid artery, then to the internal carotid artery, and finally into the proximal segment of MCA using 13 adolescent monkeys (Macaca mulatta) weighing 4.25 +/- 0.23 kg. The infarction was confirmed by magnetic resonance imaging at 3, 7, and 23 hours after MCAO. RESULTS: Local cerebral blood flow in the caudate nucleus decreased remarkably. Post-MCAO electroencephalographic monitoring showed the inhibition of electroencephalography with slow waves distinctly increasing. Neurologic evaluation demonstrated persistent deficits in the ischemic group. Neuropathologic examinations were consistent with the neurologic evaluations. CONCLUSION: The results suggested that this reversible monkey model resembled a humanstroke and should offer a reasonable alternative to craniotomy and the extrinsic ligation of the MCA in the laboratory investigation of focal reversible cerebral ischemia.
Authors: Warren G Darling; Marc A Pizzimenti; Stephanie M Hynes; Diane L Rotella; Grant Headley; Jizhi Ge; Kimberly S Stilwell-Morecraft; David W McNeal; Kathryn M Solon-Cline; Robert J Morecraft Journal: Exp Neurol Date: 2011-06-15 Impact factor: 5.330
Authors: David W Howells; Michelle J Porritt; Sarah S J Rewell; Victoria O'Collins; Emily S Sena; H Bart van der Worp; Richard J Traystman; Malcolm R Macleod Journal: J Cereb Blood Flow Metab Date: 2010-05-19 Impact factor: 6.200
Authors: Stephanie J Murphy; Jeffrey R Kirsch; Wenri Zhang; Marjorie R Grafe; G Alex West; Gregory J del Zoppo; Richard J Traystman; Patricia D Hum Journal: Comp Med Date: 2008-12 Impact factor: 0.982
Authors: Rafael Rodriguez-Mercado; Gregory D Ford; Zhenfeng Xu; Edmundo N Kraiselburd; Melween I Martinez; Vesna A Eterović; Edgar Colon; Idia V Rodriguez; Peter Portilla; Pedro A Ferchmin; Lynette Gierbolini; Maria Rodriguez-Carrasquillo; Michael D Powell; John V K Pulliam; Casey O McCraw; Alicia Gates; Byron D Ford Journal: Comp Med Date: 2012-10 Impact factor: 0.982
Authors: Cameron Rink; Greg Christoforidis; Amir Abduljalil; Marinos Kontzialis; Valerie Bergdall; Sashwati Roy; Savita Khanna; Andrew Slivka; Michael Knopp; Chandan K Sen Journal: Proc Natl Acad Sci U S A Date: 2008-09-08 Impact factor: 11.205