AIM: To assess potential function of each two-component signal transduction system in the expression of Streptococcus mutans virulence properties. METHODS AND RESULTS: For each two-component system (TCS), the histidine kinase-encoding gene was inactivated by a polymerase chain reaction (PCR)-based deletion strategy and the effects of gene disruption on the cell's ability to form biofilms, become competent, and tolerate acid, osmotic, and oxidative stress conditions were tested. Our results demonstrated that none of the mutations were lethal for S. mutans. The TCS-2 (CiaRH) is involved in biofilm formation and tolerance to environmental stresses, the TCS-3 (ScnRK-like) participates in the survival of cells at acidic pH, and the TCS-9 affects the acid tolerance response and the process of streptococcal competence development. CONCLUSIONS: Our results confirmed the physiological role of the TCS in S. mutans cellular function, in particular the SncRK-like TCS and TCS-9 as they may represent new regulatory systems than can be involved in S. mutans pathogenesis. SIGNIFICANCE AND IMPACT OF THE STUDY: Multiple TCS govern important biological parameters of S. mutans enabling its survival and persistence in the biofilm community.
AIM: To assess potential function of each two-component signal transduction system in the expression of Streptococcus mutans virulence properties. METHODS AND RESULTS: For each two-component system (TCS), the histidine kinase-encoding gene was inactivated by a polymerase chain reaction (PCR)-based deletion strategy and the effects of gene disruption on the cell's ability to form biofilms, become competent, and tolerate acid, osmotic, and oxidative stress conditions were tested. Our results demonstrated that none of the mutations were lethal for S. mutans. The TCS-2 (CiaRH) is involved in biofilm formation and tolerance to environmental stresses, the TCS-3 (ScnRK-like) participates in the survival of cells at acidic pH, and the TCS-9 affects the acid tolerance response and the process of streptococcal competence development. CONCLUSIONS: Our results confirmed the physiological role of the TCS in S. mutans cellular function, in particular the SncRK-like TCS and TCS-9 as they may represent new regulatory systems than can be involved in S. mutans pathogenesis. SIGNIFICANCE AND IMPACT OF THE STUDY: Multiple TCS govern important biological parameters of S. mutans enabling its survival and persistence in the biofilm community.
Authors: Dragana Ajdić; William M McShan; Robert E McLaughlin; Gorana Savić; Jin Chang; Matthew B Carson; Charles Primeaux; Runying Tian; Steve Kenton; Honggui Jia; Shaoping Lin; Yudong Qian; Shuling Li; Hua Zhu; Fares Najar; Hongshing Lai; Jim White; Bruce A Roe; Joseph J Ferretti Journal: Proc Natl Acad Sci U S A Date: 2002-10-23 Impact factor: 11.205
Authors: Chenggang Wu; Eduardo A Ayala; Jennifer S Downey; Justin Merritt; Steven D Goodman; Fengxia Qi Journal: J Bacteriol Date: 2010-07-16 Impact factor: 3.490
Authors: Liliana Danusia Banu; Georg Conrads; Hubert Rehrauer; Haitham Hussain; Elaine Allan; Jan R van der Ploeg Journal: Infect Immun Date: 2010-03-15 Impact factor: 3.441
Authors: J A Perry; C M Lévesque; P Suntharaligam; R W Mair; M Bu; R T Cline; S N Peterson; D G Cvitkovitch Journal: Lett Appl Microbiol Date: 2008-11 Impact factor: 2.858
Authors: Brigitte Kunze; Michael Reck; Andreas Dötsch; André Lemme; Dietmar Schummer; Herbert Irschik; Heinrich Steinmetz; Irene Wagner-Döbler Journal: BMC Microbiol Date: 2010-07-26 Impact factor: 3.605
Authors: Konstantinos Papadimitriou; Ángel Alegría; Peter A Bron; Maria de Angelis; Marco Gobbetti; Michiel Kleerebezem; José A Lemos; Daniel M Linares; Paul Ross; Catherine Stanton; Francesca Turroni; Douwe van Sinderen; Pekka Varmanen; Marco Ventura; Manuel Zúñiga; Effie Tsakalidou; Jan Kok Journal: Microbiol Mol Biol Rev Date: 2016-07-27 Impact factor: 11.056