M Zhu1, M Chen, A C Lichtler, R J O'Keefe, D Chen. 1. Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester School of Medicine, Rochester, NY 14642, USA.
Abstract
OBJECTIVE: To determine the specificity and efficiency of the tamoxifen (TM)-induced Cre-recombination in articular chondrocytes of adult Col2a1-CreER(T2) transgenic mice. METHODS: Col2a1-CreER(T2) transgenic mice were bred with Rosa26 reporter mice. Two-week-old Col2a1-CreER(T2);R26R mice were administered TM for 5 days and were sacrificed 1 and 6 months after TM induction. X-Gal staining was performed. RESULTS: Efficient Cre-recombination is achieved in adult articular chondrocytes 1 and 6 months after TM induction. CONCLUSION: Our findings demonstrate that the Col2a1-CreER(T2) transgenic mouse model is a valuable tool to target genes specifically expressed in articular chondrocytes in a temporally controlled manner in adult mice.
OBJECTIVE: To determine the specificity and efficiency of the tamoxifen (TM)-induced Cre-recombination in articular chondrocytes of adult Col2a1-CreER(T2) transgenic mice. METHODS:Col2a1-CreER(T2) transgenic mice were bred with Rosa26 reporter mice. Two-week-old Col2a1-CreER(T2);R26Rmice were administered TM for 5 days and were sacrificed 1 and 6 months after TM induction. X-Gal staining was performed. RESULTS: Efficient Cre-recombination is achieved in adult articular chondrocytes 1 and 6 months after TM induction. CONCLUSION: Our findings demonstrate that the Col2a1-CreER(T2) transgenicmouse model is a valuable tool to target genes specifically expressed in articular chondrocytes in a temporally controlled manner in adult mice.
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