Literature DB >> 17880032

A reliable quantitative method for the analysis of phenolic compounds in Brazilian propolis by reverse phase high performance liquid chromatography.

João P B de Sousa1, Paula C P Bueno, Luiz E Gregório, Ademar A da Silva Filho, Niege A J C Furtado, Márcia L de Sousa, Jairo K Bastos.   

Abstract

A sensitive and reliable RP-HPLC method was developed using a C18 CLC-ODS (M) - 4.6x250 mm(2)column and gradient elution for the analysis of phenolic compounds in propolis raw material and its products. A procedure for the extraction of phenolic compounds using aqueous ethanol (90%) with the addition of veratraldehyde as the internal standard (IS) was developed allowing to quantify ten compounds: caffeic acid, coumaric acid, ferulic acid, cinnamic acid, aromadendrin-4'-methyl ether (AME), isosakuranetin, drupanin, artepellin C, baccharin, and 2,2-dimethyl-6-carboxyethenyl-2H-1-benzopyran acid (DCBEN). The developed method gave good detection response and linearity in the range of 20.83-533.33 microg/mL.

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Year:  2007        PMID： 17880032     DOI： 10.1002/jssc.200700228

Source DB:  PubMed          Journal:  J Sep Sci        ISSN： 1615-9306            Impact factor:   3.645


  17 in total

1.  In vitro and in vivo antileishmanial activities of a Brazilian green propolis extract.

Authors:  Karina Pontin; Ademar A Da Silva Filho; Fransérgio Francisco Santos; Márcio Luís Andrade E Silva; Wilson Roberto Cunha; N P Dhammika Nanayakkara; Jairo Kenupp Bastos; Sérgio de Albuquerque
Journal:  Parasitol Res       Date:  2008-05-20       Impact factor: 2.289

2.  Stereospecific high-performance liquid chromatographic assay of isosakuranetin in rat urine.

Authors:  Karina R Vega-Villa; Connie M Remsberg; Kristy L Podelnyk; Neal M Davies
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2008-11-01       Impact factor: 3.205

3.  Propolis standardized extract (EPP-AF®), an innovative chemically and biologically reproducible pharmaceutical compound for treating wounds.

Authors:  Andresa Aparecida Berretta; Andresa Piacezzi Nascimento; Paula Carolina Pires Bueno; Mirela Mara de Oliveira Lima Leite Vaz; Juliana Maldonado Marchetti
Journal:  Int J Biol Sci       Date:  2012-03-21       Impact factor: 6.580

4.  Is propolis safe as an alternative medicine?

Authors:  Maria Graça Miguel; Maria Dulce Antunes
Journal:  J Pharm Bioallied Sci       Date:  2011-10

5.  Evaluation of Mucoadhesive Gels with Propolis (EPP-AF) in Preclinical Treatment of Candidiasis Vulvovaginal Infection.

Authors:  Andresa Aparecida Berretta; Patrícia Alves de Castro; Amanda Henriques Cavalheiro; Vanessa Silveira Fortes; Vinícius Pedro Bom; Andresa Piacezzi Nascimento; Franciane Marquele-Oliveira; Vinícius Pedrazzi; Leandra Naira Zambelli Ramalho; Gustavo Henrique Goldman
Journal:  Evid Based Complement Alternat Med       Date:  2013-08-07       Impact factor: 2.629

6.  Parasite Killing of Leishmania (V) braziliensis by Standardized Propolis Extracts.

Authors:  Jéssica Rebouças-Silva; Fabiana S Celes; Jonilson Berlink Lima; Hernane S Barud; Camila I de Oliveira; Andresa A Berretta; Valéria M Borges
Journal:  Evid Based Complement Alternat Med       Date:  2017-06-13       Impact factor: 2.629

7.  The Inhibition of Inflammasome by Brazilian Propolis (EPP-AF).

Authors:  Juliana I Hori; Dario S Zamboni; Daniel B Carrão; Gustavo Henrique Goldman; Andresa A Berretta
Journal:  Evid Based Complement Alternat Med       Date:  2013-04-16       Impact factor: 2.629

8.  Important developments in romanian propolis research.

Authors:  Liviu Al Mărghitaş; Daniel S Dezmirean; Otilia Bobiş
Journal:  Evid Based Complement Alternat Med       Date:  2013-05-30       Impact factor: 2.629

9.  Evaluation of a Propolis Water Extract Using a Reliable RP-HPLC Methodology and In Vitro and In Vivo Efficacy and Safety Characterisation.

Authors:  Bruno Alves Rocha; Paula Carolina Pires Bueno; Mirela Mara de Oliveira Lima Leite Vaz; Andresa Piacezzi Nascimento; Nathália Ursoli Ferreira; Gabriela de Padua Moreno; Marina Rezende Rodrigues; Ana Rita de Mello Costa-Machado; Edna Aparecida Barizon; Jacqueline Costa Lima Campos; Pollyanna Francielli de Oliveira; Nathália de Oliveira Acésio; Sabrina de Paula Lima Martins; Denise Crispim Tavares; Andresa Aparecida Berretta
Journal:  Evid Based Complement Alternat Med       Date:  2013-04-24       Impact factor: 2.629

10.  Seasonality Role on the Phenolics from Cultivated Baccharis dracunculifolia.

Authors:  João Paulo B de Sousa; Mateus F Leite; Renata F Jorge; Dimas O Resende; Ademar A da Silva Filho; Niege A J C Furtado; Ademilson E E Soares; Augusto C C Spadaro; Pedro Melillo de Magalhães; Jairo K Bastos
Journal:  Evid Based Complement Alternat Med       Date:  2011-06-23       Impact factor: 2.629
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