Approximate analytical time-dependent solutions to describe large-amplitude local calcium transients in the presence of buffers.

Local Ca(2+) signaling controls many neuronal functions, which is often achieved through spatial localization of Ca(2+) signals. These nanodomains are formed due to combined effects of Ca(2+) diffusion and binding to the cytoplasmic buffers. In this article we derived simple analytical expressions to describe Ca(2+) diffusion in the presence of mobile and immobile buffers. A nonlinear character of the reaction-diffusion problem was circumvented by introducing a logarithmic approximation of the concentration term. The obtained formulas reproduce free Ca(2+) levels up to 50 microM and their changes in the millisecond range. Derived equations can be useful to predict spatiotemporal profiles of large-amplitude [Ca(2+)] transients, which participate in various physiological processes.