| Literature DB >> 17869477 |
Qing Zhang1, Ping Qiu, M Gladys Arreaza, Jason S Simon, Andrei Golovko, Maureen Laverty, Galya Vassileva, Eric L Gustafson, Alberto Rojas-Triana, Loretta A Bober, Joseph A Hedrick, Frederick J Monsma, Jonathan R Greene, Marvin L Bayne, Nicholas J Murgolo.
Abstract
Mice lacking GPR103A expression display osteopenia. Analysis of mouse quantitative trait loci literature associated with bone mineral density suggested GPR103A ligand P518/Qrfp (chromosome 2qB) as a candidate osteoporosis gene. Promoter and coding regions of mouse P518/Qrfp were sequenced from genomic DNA obtained from the osteoporosis-prone strain SAMP6 and control strains SAMR1, A/J, AKR/J, BALB/c, C3H/HeJ, C57BL/6J, and DBA/2J. Four single-nucleotide polymorphisms (SNPs) were identified in only SAMP6 genomic DNA, g.-1773 T-->C, g.110 A-->G (N37S), g.188 G-->A (R63K), and g.135 T-->C (H45H). The promoter SNP generated a novel neuron-restrictive silencing factor binding site, a repressor that decreases gene expression in nonneuronal tissues. TaqMan analysis demonstrated fivefold lower P518/Qrfp liver expression in SAMP6 versus SAMR1 or C57BL/6J control strains. Tissue distribution of human, mouse, and rat P518/Qrfp and its receptors showed expression in bone and spinal cord. A direct role for P518/Qrfp function in maintaining bone mineral density is suggested.Entities:
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Year: 2007 PMID： 17869477 DOI： 10.1016/j.ygeno.2007.07.011
Source DB: PubMed Journal: Genomics ISSN： 0888-7543 Impact factor: 5.736