Literature DB >> 17828271

Three-dimensional preparation and imaging reveal intrinsic microtubule properties.

Philipp J Keller1, Francesco Pampaloni, Ernst H K Stelzer.   

Abstract

We present an experimental investigation of microtubule dynamic instability in three dimensions, based on laser light-sheet fluorescence microscopy. We introduce three-dimensional (3D) preparation of Xenopus laevis egg extracts in Teflon-based cylinders and provide algorithms for 3D image processing. Our approach gives experimental access to the intrinsic dynamic properties of microtubules and to microtubule population statistics in single asters. We obtain evidence for a stochastic nature of microtubule pausing.

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Mesh：

Year:  2007        PMID： 17828271     DOI： 10.1038/nmeth1087

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  12 in total

1.  Fast fluorescence microscopy for imaging the dynamics of embryonic development.

Authors:  Julien Vermot; Scott E Fraser; Michael Liebling
Journal:  HFSP J       Date:  2008-05-13

2.  Light sheet-based fluorescence microscopy: more dimensions, more photons, and less photodamage.

Authors:  Emmanuel G Reynaud; Uros Krzic; Klaus Greger; Ernst H K Stelzer
Journal:  HFSP J       Date:  2008-09-15

3.  Quantitative imaging of cell dynamics in mouse embryos using light-sheet microscopy.

Authors:  Ryan S Udan; Victor G Piazza; Chih-Wei Hsu; Anna-Katerina Hadjantonakis; Mary E Dickinson
Journal:  Development       Date:  2014-10-24       Impact factor: 6.868

4.  Lateral assembly of N-cadherin drives tissue integrity by stabilizing adherens junctions.

Authors:  S Garg; S C Fischer; E M Schuman; E H K Stelzer
Journal:  J R Soc Interface       Date:  2015-03-06       Impact factor: 4.118

5.  Three-dimensional microtubule behavior in Xenopus egg extracts reveals four dynamic states and state-dependent elastic properties.

Authors:  Philipp J Keller; Francesco Pampaloni; Gianluca Lattanzi; Ernst H K Stelzer
Journal:  Biophys J       Date:  2008-04-25       Impact factor: 4.033

6.  Quantitative image analysis identifies pVHL as a key regulator of microtubule dynamic instability.

Authors:  Claudio R Thoma; Alexandre Matov; Katrin L Gutbrodt; Christian R Hoerner; Zlatko Smole; Wilhelm Krek; Gaudenz Danuser
Journal:  J Cell Biol       Date:  2010-09-20       Impact factor: 10.539

Review 7.  Molecular mechanisms underlying microtubule growth dynamics.

Authors:  Joseph M Cleary; William O Hancock
Journal:  Curr Biol       Date:  2021-05-24       Impact factor: 10.900

8.  A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics.

Authors:  Etai Sapoznik; Bo-Jui Chang; Jaewon Huh; Robert J Ju; Evgenia V Azarova; Theresa Pohlkamp; Erik S Welf; David Broadbent; Alexandre F Carisey; Samantha J Stehbens; Kyung-Min Lee; Arnaldo Marín; Ariella B Hanker; Jens C Schmidt; Carlos L Arteaga; Bin Yang; Yoshihiko Kobayashi; Purushothama Rao Tata; Rory Kruithoff; Konstantin Doubrovinski; Douglas P Shepherd; Alfred Millett-Sikking; Andrew G York; Kevin M Dean; Reto P Fiolka
Journal:  Elife       Date:  2020-11-12       Impact factor: 8.140

9.  In vivo imaging of zebrafish embryogenesis.

Authors:  Philipp J Keller
Journal:  Methods       Date:  2013-03-21       Impact factor: 3.608

10.  High-speed imaging of amoeboid movements using light-sheet microscopy.

Authors:  Daisuke Takao; Atsushi Taniguchi; Takaaki Takeda; Seiji Sonobe; Shigenori Nonaka
Journal:  PLoS One       Date:  2012-12-05       Impact factor: 3.240
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