Literature DB >> 17827539

An improved method for single step purification of metagenomic DNA.

Pushpender K Sharma1, Neena Capalash, Jagdeep Kaur.   

Abstract

An improved method for purification of intact metagenomic DNA from soil has been developed using Q-Sepharose, which purified the DNA from phenolic and humic acid contaminants in a single step. The entire procedure for purification took only 45 min. A total of 81% of DNA was recovered after purification and there was 84% reduction in humic acid contents. The purified DNA was readily digested with restriction enzymes and can be further used for molecular applications.

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Year:  2007        PMID： 17827539     DOI： 10.1007/s12033-007-0015-3

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.860


  14 in total

1.  Extraction and purification of DNA in rhizosphere soil samples for PCR-DGGE analysis of bacterial consortia.

Authors:  R Maarit Niemi; I Heiskanen; K Wallenius; K Lindström
Journal:  J Microbiol Methods       Date:  2001-07       Impact factor: 2.363

2.  DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community.

Authors:  William E Holben; Janet K Jansson; Barry K Chelm; James M Tiedje
Journal:  Appl Environ Microbiol       Date:  1988-03       Impact factor: 4.792

3.  Isolation of novel lipolytic genes from uncultured bacteria of pond water.

Authors:  Ravi Ranjan; Amit Grover; Raj Kishor Kapardar; Rakesh Sharma
Journal:  Biochem Biophys Res Commun       Date:  2005-09-16       Impact factor: 3.575

4.  Direct extraction of microbial community DNA from humified upland soils.

Authors:  C D Clegg; K Ritz; B S Griffiths
Journal:  Lett Appl Microbiol       Date:  1997-07       Impact factor: 2.858

5.  Bacterial diversity of a Carolina bay as determined by 16S rRNA gene analysis: confirmation of novel taxa.

Authors:  M G Wise; J V McArthur; L J Shimkets
Journal:  Appl Environ Microbiol       Date:  1997-04       Impact factor: 4.792

6.  DNA amplification to enhance detection of genetically engineered bacteria in environmental samples.

Authors:  R J Steffan; R M Atlas
Journal:  Appl Environ Microbiol       Date:  1988-09       Impact factor: 4.792

7.  Comparative analysis of environmental DNA extraction and purification methods from different humic acid-rich soils.

Authors:  F M Lakay; A Botha; B A Prior
Journal:  J Appl Microbiol       Date:  2007-01       Impact factor: 3.772

8.  Rapid extraction and purification of environmental DNA for molecular cloning applications and molecular diversity studies.

Authors:  D A Santosa
Journal:  Mol Biotechnol       Date:  2001-01       Impact factor: 2.695

9.  Quantitative cell lysis of indigenous microorganisms and rapid extraction of microbial DNA from sediment.

Authors:  M I Moré; J B Herrick; M C Silva; W C Ghiorse; E L Madsen
Journal:  Appl Environ Microbiol       Date:  1994-05       Impact factor: 4.792

10.  Methods for microbial DNA extraction from soil for PCR amplification.

Authors:  C Yeates; M R Gillings; A D Davison; N Altavilla; D A Veal
Journal:  Biol Proced Online       Date:  1998-05-14       Impact factor: 3.244
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  19 in total

1.  Cloning, expression and characterization of a metagenome derived thermoactive/thermostable pectinase.

Authors:  Rajvinder Singh; Samriti Dhawan; Kashmir Singh; Jagdeep Kaur
Journal:  Mol Biol Rep       Date:  2012-06-19       Impact factor: 2.316

2.  Extraction of mRNA from soil.

Authors:  Carsten Mettel; Yongkyu Kim; Pravin Malla Shrestha; Werner Liesack
Journal:  Appl Environ Microbiol       Date:  2010-07-09       Impact factor: 4.792

3.  Bio-Prospecting Laccases in the Bacterial Diversity of Activated Sludge From Pulp and Paper Industry.

Authors:  Vijaya Gupta; Neena Capalash; Naveen Gupta; Prince Sharma
Journal:  Indian J Microbiol       Date:  2016-09-26       Impact factor: 2.461

4.  A simple silica-based method for metagenomic DNA extraction from soil and sediments.

Authors:  R Rojas-Herrera; J Narváez-Zapata; M Zamudio-Maya; M E Mena-Martínez
Journal:  Mol Biotechnol       Date:  2008-03-29       Impact factor: 2.695

5.  Characterization of a thermostable lipase showing loss of secondary structure at ambient temperature.

Authors:  Pushpender Kumar Sharma; Kashmir Singh; Ranvir Singh; Neena Capalash; Azmat Ali; Owais Mohammad; Jagdeep Kaur
Journal:  Mol Biol Rep       Date:  2011-06-16       Impact factor: 2.316

6.  An improved method suitable for isolation of high-quality metagenomic DNA from diverse soils.

Authors:  Sumit Kumar Verma; Himanshi Singh; Prakash C Sharma
Journal:  3 Biotech       Date:  2017-06-29       Impact factor: 2.406

7.  Optimization of high-yielding protocol for DNA extraction from the forest rhizosphere microbes.

Authors:  Sadam D V Satyanarayana; M S R Krishna; Pindi Pavan Kumar
Journal:  3 Biotech       Date:  2017-05-29       Impact factor: 2.406

8.  Characterization and evolution of a metagenome-derived lipase towards enhanced enzyme activity and thermostability.

Authors:  Rakesh Kumar; Monika Sharma; Ranvir Singh; Jagdeep Kaur
Journal:  Mol Cell Biochem       Date:  2012-10-27       Impact factor: 3.396

9.  Chicken or the Egg: Microbial Alterations in Biopsy Samples of Patients with Oral Potentially Malignant Disorders.

Authors:  Gabor Decsi; Jozsef Soki; Bernadett Pap; Gabriella Dobra; Maria Harmati; Sandor Kormondi; Tibor Pankotai; Gabor Braunitzer; Janos Minarovits; Istvan Sonkodi; Edit Urban; Istvan Balazs Nemeth; Katalin Nagy; Krisztina Buzas
Journal:  Pathol Oncol Res       Date:  2018-07-27       Impact factor: 3.201

10.  Mining the metagenome of activated biomass of an industrial wastewater treatment plant by a novel method.

Authors:  Nandita Sharma; Himgouri Tanksale; Atya Kapley; Hemant J Purohit
Journal:  Indian J Microbiol       Date:  2012-03-25       Impact factor: 2.461
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