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Literature DB >> 1782574

Effects of inositol trisphosphate on calcium mobilization in bone cells.

R Falsafi¹, D N Tatakis, S Hagel-Bradway, R Dziak.

Abstract

The effect of inositol 1,4,5 trisphosphate (IP3) on calcium mobilization was studied in human osteosarcoma lines, Saos-2 and G292, as well as isolated rat osteoblastic and osteoclastic cells. Cells were permeabilized with saponin and calcium mobilization was studied with the fluorescent dye, fura-2 in a recording spectrofluorometer. IP3 (10 microM) increased calcium release in all cell types studied. The effect was dependent on ATP and occurred in the presence of mitochondrial inhibitors. The effect was not seen with inositol 1-phosphate (IP) or inositol 1,4-diphosphate (IP2). Inositol 1,3,4,5 tetrakisphosphate (IP4) appeared to elicit a decrease in the calcium released. Depletion of the intracellular pool with the calcium ionophore, ionomycin, as well as incubation with the inhibitor of intracellular calcium mobilization, TMB-8, obliterated the IP3 effect. The results are consistent with the hypothesis that increases in IP3 can cause a rapid elevation of bone cell cytosolic calcium.

Entities: Chemical Disease Gene Species

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Year: 1991 PMID： 1782574 DOI： 10.1007/bf02556256

Source DB: PubMed Journal: Calcif Tissue Int ISSN： 0171-967X Impact factor: 4.333

41 in total

Review 1. Inositol trisphosphate and diacylglycerol: two interacting second messengers.

Authors: M J Berridge
Journal: Annu Rev Biochem Date: 1987 Impact factor: 23.643

2. Formation of bone by isolated, cultured osteoblasts in millipore diffusion chambers.

Authors: D J Simmons; G N Kent; R L Jilka; D M Scott; M Fallon; D V Cohn
Journal: Calcif Tissue Int Date: 1982-05 Impact factor: 4.333

3. Control of cytosolic free calcium in rat and chicken osteoclasts. The role of extracellular calcium and calcitonin.

Authors: A Malgaroli; J Meldolesi; A Z Zallone; A Teti
Journal: J Biol Chem Date: 1989-08-25 Impact factor: 5.157

4. Thrombin's effects on osteoblastic cells. I. Cytosolic calcium and phosphoinositides.

Authors: D N Tatakis; C Dolce; R Dziak
Journal: Biochem Biophys Res Commun Date: 1989-10-16 Impact factor: 3.575

5. The effect of inositol trisphosphate on Ca2+ fluxes in insulin-secreting tumor cells.

Authors: S K Joseph; R J Williams; B E Corkey; F M Matschinsky; J R Williamson
Journal: J Biol Chem Date: 1984-11-10 Impact factor: 5.157

6. Inositol 1,4,5-trisphosphate mobilizes intracellular Ca2+ from permeabilized insulin-secreting cells.

Authors: T J Biden; M Prentki; R F Irvine; M J Berridge; C B Wollheim
Journal: Biochem J Date: 1984-10-15 Impact factor: 3.857

7. Isolation of mitochondria from ascites tumor cells permeabilized with digitonin.

Authors: R W Moreadith; G Fiskum
Journal: Anal Biochem Date: 1984-03 Impact factor: 3.365

8. Inositol trisphosphate and calcium mobilization.

Authors: M J Berridge
Journal: Ciba Found Symp Date: 1986

9. A two-receptor model for the action of parathyroid hormone on osteoblasts: a role for intracellular free calcium and cAMP.

Authors: C W Löwik; J P van Leeuwen; J M van der Meer; J K van Zeeland; B A Scheven; M P Herrmann-Erlee
Journal: Cell Calcium Date: 1985-08 Impact factor: 6.817

10. Parathyroid hormone-activated calcium channels in an osteoblast-like clonal osteosarcoma cell line. cAMP-dependent and cAMP-independent calcium channels.

Authors: D T Yamaguchi; T J Hahn; A Iida-Klein; C R Kleeman; S Muallem
Journal: J Biol Chem Date: 1987-06-05 Impact factor: 5.157

7 in total

Review 1. Calcium signaling in osteoclasts.

Authors: Sung-Yong Hwang; James W Putney
Journal: Biochim Biophys Acta Date: 2010-11-11

Effects of inositol trisphosphate on calcium mobilization in bone cells.

Review 1. Inositol trisphosphate and diacylglycerol: two interacting second messengers.

2. Formation of bone by isolated, cultured osteoblasts in millipore diffusion chambers.

3. Control of cytosolic free calcium in rat and chicken osteoclasts. The role of extracellular calcium and calcitonin.

4. Thrombin's effects on osteoblastic cells. I. Cytosolic calcium and phosphoinositides.

5. The effect of inositol trisphosphate on Ca2+ fluxes in insulin-secreting tumor cells.

6. Inositol 1,4,5-trisphosphate mobilizes intracellular Ca2+ from permeabilized insulin-secreting cells.

7. Isolation of mitochondria from ascites tumor cells permeabilized with digitonin.

8. Inositol trisphosphate and calcium mobilization.

9. A two-receptor model for the action of parathyroid hormone on osteoblasts: a role for intracellular free calcium and cAMP.

10. Parathyroid hormone-activated calcium channels in an osteoblast-like clonal osteosarcoma cell line. cAMP-dependent and cAMP-independent calcium channels.

Review 1. Calcium signaling in osteoclasts.

2. Pharmacological inhibition of intracellular calcium release blocks acid-induced bone resorption.

3. Dye and electric coupling between osteoblast-like cells in culture.

Review 4. The roles of Orai and Stim in bone health and disease.

Review 5. Calcium signalling and calcium transport in bone disease.

6. Necessity of inositol (1,4,5)-trisphosphate receptor 1 and mu-calpain in NO-induced osteoclast motility.

Review 7. Osteoclastic differentiation and function regulated by old and new pathways.