INTRODUCTION: Autoinducer-2 (AI-2) is a widespread communication-signal molecule that allows bacteria to sense and react to environmental factors. In some streptococci AI-2 is reported to be involved in virulence expression and biofilm formation. It has earlier been shown that the alga Delisea pulchra produces bromated furanones, which prevent bacterial colonization of the algae. METHODS AND RESULTS: We have previously published a novel and simple synthesis of (Z)-5-bromomethylene-2(5H)-furanone. In this study we showed that our synthesized furanone inhibited biofilm formation and bioluminescence induction by Streptococcus anginosus, Streptococcus intermedius, and Streptococcus mutans, as well as bioluminescence induction by Vibrio harveyi BB152. CONCLUSION: We suggest that the effect is linked to interference with the AI-2 signaling pathway because adding furanone to the medium had no effect on the ability of the AI-2-defective S. anginosus luxS and S. intermedius luxS mutants to form biofilms.
INTRODUCTION: Autoinducer-2 (AI-2) is a widespread communication-signal molecule that allows bacteria to sense and react to environmental factors. In some streptococci AI-2 is reported to be involved in virulence expression and biofilm formation. It has earlier been shown that the alga Delisea pulchra produces bromated furanones, which prevent bacterial colonization of the algae. METHODS AND RESULTS: We have previously published a novel and simple synthesis of (Z)-5-bromomethylene-2(5H)-furanone. In this study we showed that our synthesized furanone inhibited biofilm formation and bioluminescence induction by Streptococcus anginosus, Streptococcus intermedius, and Streptococcus mutans, as well as bioluminescence induction by Vibrio harveyi BB152. CONCLUSION: We suggest that the effect is linked to interference with the AI-2 signaling pathway because adding furanone to the medium had no effect on the ability of the AI-2-defective S. anginosus luxS and S. intermedius luxS mutants to form biofilms.
Authors: Tianzhu Zang; Bobby W K Lee; Lisa M Cannon; Kathryn A Ritter; Shujia Dai; Dacheng Ren; Thomas K Wood; Zhaohui Sunny Zhou Journal: Bioorg Med Chem Lett Date: 2009-09-03 Impact factor: 2.823
Authors: Joost C A Janssens; Hans Steenackers; Stijn Robijns; Edith Gellens; Jeremy Levin; Hui Zhao; Kim Hermans; David De Coster; Tine L Verhoeven; Kathleen Marchal; Jos Vanderleyden; Dirk E De Vos; Sigrid C J De Keersmaecker Journal: Appl Environ Microbiol Date: 2008-09-12 Impact factor: 4.792