| Literature DB >> 17803514 |
Abstract
Immunoproteomic approaches were undertaken to study the immunogenicity of the membrane-associated proteins of the Streptococcus suis type 2 (SS2) China vaccine strain HA9801. The membrane-associated proteins were enriched using the Triton X-114 extraction protocol and were analysed by two-dimensional gel electrophoresis (2-DE) and subsequent immunoblotting using the hyperimmune serum of SS2-HA9801-immunized specific pathogen free (SPF) minipigs. A total of 11 proteins were recognized, and the corresponding spots on a duplicate gel were excised and identified by MALDI-TOF MS.Entities:
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Year: 2007 PMID: 17803514 DOI: 10.1111/j.1863-2378.2007.01056.x
Source DB: PubMed Journal: Zoonoses Public Health ISSN: 1863-1959 Impact factor: 2.702