Literature DB >> 17786029

Chromosomal mapping of the GFP-LC3 transgene in GFP-LC3 mice.

Akiko Kuma1, Noboru Mizushima.   

Abstract

GFP-LC3 mice are widely used to monitor autophagy. Here, we report chromosomal mapping of the GFP.LC3 transgene in this mouse model. The transgene was located in a very distal region of chromosome 2, immediately upstream of a putative open reading frame that is thought to be a part of a pseudogene. With this information, we were able to generate a PCR system to easily determine zygosity. Homozygous GFP-LC3 mice (GFP-LC3(tg/tg)) were healthy, fertile and did not show an abnormal phenotype. Thus, GFP-LC3 integration in this model appears not to affect functions of other genes, justifying use of this mouse model.

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Year:  2007        PMID： 17786029     DOI： 10.4161/auto.4846

Source DB:  PubMed          Journal:  Autophagy        ISSN： 1554-8627            Impact factor:   16.016


  21 in total

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