Literature DB >> 1777482

Description of a baby machine for Saccharomyces cerevisiae.

C E Helmstetter1.   

Abstract

A method for the continuous withdrawal of newly formed daughter cells from a growing population of Saccharomyces cerevisiae is described. An exponential-phase culture of cells was immobilized onto a surface and then flushed continuously with culture medium. Upon division of a cell in the immobilized population, the mother cell remained attached to the surface and the daughter cell was released. The method can be applied to research on the cell cycle, the segregation of components between cells, and cellular aging.

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Year:  1991        PMID： 1777482

Source DB:  PubMed          Journal:  New Biol        ISSN： 1043-4674


  10 in total

1.  The Escherichia coli baby cell column: a novel cell synchronization method provides new insight into the bacterial cell cycle.

Authors:  David Bates; Jessica Epstein; Erik Boye; Karen Fahrner; Howard Berg; Nancy Kleckner
Journal:  Mol Microbiol       Date:  2005-07       Impact factor: 3.501

2.  Technology for cell cycle research with unstressed steady-state cultures.

Authors:  Valerie S Lebleu; Maureen Thornton; Steven R Gonda; Charles E Helmstetter
Journal:  Cytotechnology       Date:  2006-11-21       Impact factor: 2.058

3.  Improved bacterial baby machine: application to Escherichia coli K-12.

Authors:  C E Helmstetter; C Eenhuis; P Theisen; J Grimwade; A C Leonard
Journal:  J Bacteriol       Date:  1992-06       Impact factor: 3.490

4.  Gravity and the orientation of cell division.

Authors:  C E Helmstetter
Journal:  Proc Natl Acad Sci U S A       Date:  1997-09-16       Impact factor: 11.205

Review 5.  Review of methods to probe single cell metabolism and bioenergetics.

Authors:  Andreas E Vasdekis; Gregory Stephanopoulos
Journal:  Metab Eng       Date:  2014-10-31       Impact factor: 9.783

6.  Measurement of the volume growth rate of single budding yeast with the MOSFET-based microfluidic Coulter counter.

Authors:  Jiashu Sun; Chris C Stowers; Erik M Boczko; Deyu Li
Journal:  Lab Chip       Date:  2010-08-18       Impact factor: 6.799

7.  Microfluidic device for automated synchronization of bacterial cells.

Authors:  Seth M Madren; Michelle D Hoffman; Pamela J B Brown; David T Kysela; Yves V Brun; Stephen C Jacobson
Journal:  Anal Chem       Date:  2012-10-03       Impact factor: 6.986

8.  Candida albicans, a distinctive fungal model for cellular aging study.

Authors:  Xiao-Hong Fu; Fei-Long Meng; Yan Hu; Jin-Qiu Zhou
Journal:  Aging Cell       Date:  2008-08-01       Impact factor: 9.304

Review 9.  Longevity regulation in Saccharomyces cerevisiae: linking metabolism, genome stability, and heterochromatin.

Authors:  Kevin J Bitterman; Oliver Medvedik; David A Sinclair
Journal:  Microbiol Mol Biol Rev       Date:  2003-09       Impact factor: 11.056

10.  Membrane-elution analysis of content of cyclins A, B1, and E during the unperturbed mammalian cell cycle.

Authors:  Stephen Cooper; Michelle Paulsen; Mats Ljungman; Dang Vu-Phan; Duyang Kim; Mariam Gonzalez-Hernandez
Journal:  Cell Div       Date:  2007-09-24       Impact factor: 5.130
  10 in total

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