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Literature DB >> 17768358

Crystallization and preliminary X-ray diffraction analysis of the small subunit of the heterodimeric restriction endonuclease R.BspD6I.

Galina S Kachalova¹, Alfiya K Yunusova, Rimma I Artyukh, Eugeny A Rogulin, Tatyana A Perevyazova, Ludmila A Zheleznaya, Nickolay I Matvienko, Hans D Bartunik.

Abstract

The heterodimeric restriction endonuclease R.BspD6I is composed of a small subunit with a cleavage site and a large subunit, containing a recognition domain and a cleavage domain, that may function separately as a monomeric nicking endonuclease. Here, the crystallization of the small subunit and diffraction data collection to 1.5 A resolution are reported.

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Year: 2007 PMID： 17768358 PMCID： PMC2376331 DOI： 10.1107/S174430910704016X

Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091

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References

7 in total

1. Cloning and sequencing of the gene of site-specific nickase N.BspD6I.

Authors: T A Perevyazova; E A Rogulin; L A Zheleznaya; N I Matvienko
Journal: Biochemistry (Mosc) Date: 2003-09 Impact factor: 2.487

2. ARP/wARP and automatic interpretation of protein electron density maps.

Authors: Richard J Morris; Anastassis Perrakis; Victor S Lamzin
Journal: Methods Enzymol Date: 2003 Impact factor: 1.600

3. Site-specific DNA-nicking mutants of the heterodimeric restriction endonuclease R.BbvCI.

Authors: Daniel F Heiter; Keith D Lunnen; Geoffrey G Wilson
Journal: J Mol Biol Date: 2005-05-06 Impact factor: 5.469

4. Nickase and a protein encoded by an open reading frame downstream from the nickase BspD6I gene form a restriction endonuclease complex.

Authors: A K Yunusova; E A Rogulin; R I Artyukh; L A Zheleznaya; N I Matvienko
Journal: Biochemistry (Mosc) Date: 2006-07 Impact factor: 2.487

Crystallization and preliminary X-ray diffraction analysis of the small subunit of the heterodimeric restriction endonuclease R.BspD6I.

1. Cloning and sequencing of the gene of site-specific nickase N.BspD6I.

2. ARP/wARP and automatic interpretation of protein electron density maps.

3. Site-specific DNA-nicking mutants of the heterodimeric restriction endonuclease R.BbvCI.

4. Nickase and a protein encoded by an open reading frame downstream from the nickase BspD6I gene form a restriction endonuclease complex.

5. Crystallization and preliminary crystallographic analysis of the site-specific DNA nickase Nb.BspD6I.

6. Cloning and analysis of the four genes coding for Bpu10I restriction-modification enzymes.

7. Solvent content of protein crystals.