Literature DB >> 16511033

Crystallization and preliminary crystallographic analysis of the site-specific DNA nickase Nb.BspD6I.

Galina S Kachalova1, Eugeny A Rogulin, Rimma I Artyukh, Tatyana A Perevyazova, Ludmila A Zheleznaya, Nickolay I Matvienko, Hans D Bartunik.   

Abstract

Crystals of site-specific DNA nickase Nb.BspD6I (of molecular weight 70.8 kDa) have been grown at 291 K using PEG 8000 as precipitant. The diffraction pattern of the crystal extends to 3.3 A resolution at 100 K. The crystal belongs to space group P2(1), with unit-cell parameters a = 57.76, b = 90.67, c = 71.71, beta = 110.1 degrees. There is one molecule in the asymmetric unit and the solvent content is estimated to be 53% by volume.

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Year:  2005        PMID： 16511033      PMCID： PMC1952280          DOI： 10.1107/S174430910500309X

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  16 in total

1.  Characterization of the specific DNA nicking activity of restriction endonuclease N.BstNBI.

Authors:  R D Morgan; C Calvet; M Demeter; R Agra; H Kong
Journal:  Biol Chem       Date:  2000-11       Impact factor: 3.915

2.  A DNA computing readout operation based on structure-specific cleavage.

Authors:  L Wang; J G Hall; M Lu; Q Liu; L M Smith
Journal:  Nat Biotechnol       Date:  2001-11       Impact factor: 54.908

3.  Engineering a nicking endonuclease N.AlwI by domain swapping.

Authors:  Y Xu; K D Lunnen; H Kong
Journal:  Proc Natl Acad Sci U S A       Date:  2001-10-30       Impact factor: 11.205

4.  Artificial site-specific DNA-nicking system based on common restriction enzyme assisted by PNA openers.

Authors:  Heiko Kuhn; Yongbo Hu; Maxim D Frank-Kamenetskii; Vadim V Demidov
Journal:  Biochemistry       Date:  2003-05-06       Impact factor: 3.162

5.  The isolation of strand-specific nicking endonucleases from a randomized SapI expression library.

Authors:  James C Samuelson; Zhenyu Zhu; Shuang-yong Xu
Journal:  Nucleic Acids Res       Date:  2004-07-09       Impact factor: 16.971

6.  Plasmid pRARE as a vector for cloning to construct a superproducer of the site-specific nickase N.BspD6I.

Authors:  E A Rogulin; T A Perevyazova; L A Zheleznaya; N I Matvienko
Journal:  Biochemistry (Mosc)       Date:  2004-10       Impact factor: 2.487

7.  [N.BstSE--a site-specific nickase from Bacillus stearothermophilus SE-589].

Authors:  M A Abdurashitov; O A Belitchenko; A V Shevchenko; S Kh Degtiarev
Journal:  Mol Biol (Mosk)       Date:  1996 Nov-Dec

8.  5'-3' exonucleases in phosphorothioate-based oligonucleotide-directed mutagenesis.

Authors:  J R Sayers; W Schmidt; F Eckstein
Journal:  Nucleic Acids Res       Date:  1988-02-11       Impact factor: 16.971

9.  Site-specific nickase from bacillus species strain d6.

Authors:  L A Zheleznaya; T A Perevyazova; D V Alzhanova; N I Matvienko
Journal:  Biochemistry (Mosc)       Date:  2001-09       Impact factor: 2.487

10.  Detection of Mycobacterium tuberculosis DNA with thermophilic strand displacement amplification and fluorescence polarization.

Authors:  G T Walker; C P Linn
Journal:  Clin Chem       Date:  1996-10       Impact factor: 8.327
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  2 in total

1.  Crystallization and preliminary X-ray diffraction analysis of the small subunit of the heterodimeric restriction endonuclease R.BspD6I.

Authors:  Galina S Kachalova; Alfiya K Yunusova; Rimma I Artyukh; Eugeny A Rogulin; Tatyana A Perevyazova; Ludmila A Zheleznaya; Nickolay I Matvienko; Hans D Bartunik
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2007-08-31

2.  Structural and evolutionary classification of Type II restriction enzymes based on theoretical and experimental analyses.

Authors:  Jerzy Orlowski; Janusz M Bujnicki
Journal:  Nucleic Acids Res       Date:  2008-05-02       Impact factor: 16.971
  2 in total

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