Literature DB >> 17720879

A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells.

Peter T Nelson1, Mariangels De Planell-Saguer, Stella Lamprinaki, Marianthi Kiriakidou, Paul Zhang, Una O'Doherty, Zissimos Mourelatos.   

Abstract

Argonaute (Ago) proteins bind to microRNA (miRNAs) and short interfering RNAs (siRNAs) and form the core components of effector complexes that mediate miRNA and siRNA function. Currently, there is a paucity of reliable antibodies against mammalian Ago proteins, thus precluding studies of endogenous Ago proteins from tissues. Here we report the development of 2A8, a novel anti-Ago monoclonal antibody that recognizes human and mouse Ago proteins and efficiently immunoprecipitates miRNAs. We report the characterization of 2A8 and its use to clone miRNAs from human brain and from preparations of human polymorphonuclear leukocytes (neutrophils), which revealed a prevalent miRNA with unusual features.

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Year:  2007        PMID： 17720879      PMCID： PMC1986805          DOI： 10.1261/rna.646007

Source DB:  PubMed          Journal:  RNA        ISSN： 1355-8382            Impact factor:   4.942


  29 in total

Review 1.  The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis.

Authors:  Michelle A Carmell; Zhenyu Xuan; Michael Q Zhang; Gregory J Hannon
Journal:  Genes Dev       Date:  2002-11-01       Impact factor: 11.361

Review 2.  Dicing and slicing: the core machinery of the RNA interference pathway.

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Review 3.  Noncoding RNAs in the mammalian central nervous system.

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4.  miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs.

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Journal:  Genes Dev       Date:  2002-03-15       Impact factor: 11.361

5.  Analysis of short RNAs in the malaria parasite and its red blood cell host.

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Journal:  FEBS Lett       Date:  2006-09-05       Impact factor: 4.124

Review 6.  Radixin: cytoskeletal adopter and signaling protein.

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Journal:  Int J Biochem Cell Biol       Date:  2004-11       Impact factor: 5.085

7.  MicroRNA expression dynamics during murine and human erythroid differentiation.

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Journal:  Exp Hematol       Date:  2007-07       Impact factor: 3.084

Review 8.  Mighty Piwis defend the germline against genome intruders.

Authors:  Kathryn A O'Donnell; Jef D Boeke
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9.  Identification of eight members of the Argonaute family in the human genome.

Authors:  Takashi Sasaki; Aiko Shiohama; Shinsei Minoshima; Nobuyoshi Shimizu
Journal:  Genomics       Date:  2003-09       Impact factor: 5.736

10.  Identification of clustered microRNAs using an ab initio prediction method.

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Journal:  BMC Bioinformatics       Date:  2005-11-07       Impact factor: 3.169
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  76 in total

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Authors:  Kasandra J Riley; Gabrielle S Rabinowitz; Therese A Yario; Joseph M Luna; Robert B Darnell; Joan A Steitz
Journal:  EMBO J       Date:  2012-03-30       Impact factor: 11.598

2.  Demonstrating polymorphic miRNA-mediated gene regulation in vivo: application to the g+6223G->A mutation of Texel sheep.

Authors:  Haruko Takeda; Carole Charlier; Frédéric Farnir; Michel Georges
Journal:  RNA       Date:  2010-08-02       Impact factor: 4.942

3.  Individual microRNAs (miRNAs) display distinct mRNA targeting "rules".

Authors:  Wang-Xia Wang; Bernard R Wilfred; Kevin Xie; Mary H Jennings; Yanling Hu Hu; Arnold J Stromberg; Peter T Nelson
Journal:  RNA Biol       Date:  2010 May-Jun       Impact factor: 4.652

4.  Complexity of the microRNA repertoire revealed by next-generation sequencing.

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Journal:  RNA       Date:  2010-09-28       Impact factor: 4.942

5.  Site-specific crosslinking of human microRNPs to RNA targets.

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6.  A p21-ZEB1 complex inhibits epithelial-mesenchymal transition through the microRNA 183-96-182 cluster.

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Journal:  Mol Cell Biol       Date:  2013-11-25       Impact factor: 4.272

7.  Alternative RISC assembly: binding and repression of microRNA-mRNA duplexes by human Ago proteins.

Authors:  Maja M Janas; Bingbing Wang; Abigail S Harris; Mike Aguiar; Jonathan M Shaffer; Yerramilli V B K Subrahmanyam; Mark A Behlke; Kai W Wucherpfennig; Steven P Gygi; Etienne Gagnon; Carl D Novina
Journal:  RNA       Date:  2012-09-27       Impact factor: 4.942

8.  Poly(A)-specific ribonuclease mediates 3'-end trimming of Argonaute2-cleaved precursor microRNAs.

Authors:  Mayuko Yoda; Daniel Cifuentes; Natsuko Izumi; Yuriko Sakaguchi; Tsutomu Suzuki; Antonio J Giraldez; Yukihide Tomari
Journal:  Cell Rep       Date:  2013-10-24       Impact factor: 9.423

9.  Mutational inactivation of herpes simplex virus 1 microRNAs identifies viral mRNA targets and reveals phenotypic effects in culture.

Authors:  Omar Flores; Sanae Nakayama; Adam W Whisnant; Hassan Javanbakht; Bryan R Cullen; David C Bloom
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10.  Translocation of sickle cell erythrocyte microRNAs into Plasmodium falciparum inhibits parasite translation and contributes to malaria resistance.

Authors:  Gregory LaMonte; Nisha Philip; Joseph Reardon; Joshua R Lacsina; William Majoros; Lesley Chapman; Courtney D Thornburg; Marilyn J Telen; Uwe Ohler; Christopher V Nicchitta; Timothy Haystead; Jen-Tsan Chi
Journal:  Cell Host Microbe       Date:  2012-08-16       Impact factor: 21.023
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