BACKGROUND: Endothelial dysfunction may be related to adverse effects of some dietary fatty acids (FAs). Although in vitro studies have failed to show consistent findings, this may reflect the diverse experimental protocols employed and the limited range of FAs and end points studied. AIMS: To investigate the effect of dietary FA type (saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids), concentration, incubation time and cell stimulation state, on a broad spectrum of endothelial inflammatory gene expression. METHODS: Using human umbilical vein endothelial cells, with and without stimulation (+/-10 ng/ml TNFalpha), the effects of arachidonic (AA), docosahexaenoic (DHA), eicosapentaenoic (EPA), linoleic (LA), oleic (OA) and palmitic acids (PA) (10, 25 and 100 microM), on the expression of genes encoding a number of inflammatory proteins and transcription factors were assessed by quantitative real time RT-PCR. RESULTS: Individual FAs differentially affect endothelial inflammatory gene expression in a gene-specific manner. EPA, LA and OA significantly up-regulated MCP-1 gene expression compared to AA (p = 0.001, 0.013, 0.008, respectively) and DHA (p < 0.0005, = 0.004, 0.002, respectively). Furthermore, cell stimulation state and FA incubation time significantly influenced reported FA effects on gene expression. CONCLUSION: The comparative effects of saturated, monounsaturated, n-6 and n-3 polyunsaturated FAs on endothelial gene expression depend on the specific FA investigated, its length of incubation, cell stimulation state and the gene investigated. These findings may explain existing disparity in the literature.
BACKGROUND: Endothelial dysfunction may be related to adverse effects of some dietary fatty acids (FAs). Although in vitro studies have failed to show consistent findings, this may reflect the diverse experimental protocols employed and the limited range of FAs and end points studied. AIMS: To investigate the effect of dietary FA type (saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids), concentration, incubation time and cell stimulation state, on a broad spectrum of endothelial inflammatory gene expression. METHODS: Using human umbilical vein endothelial cells, with and without stimulation (+/-10 ng/ml TNFalpha), the effects of arachidonic (AA), docosahexaenoic (DHA), eicosapentaenoic (EPA), linoleic (LA), oleic (OA) and palmitic acids (PA) (10, 25 and 100 microM), on the expression of genes encoding a number of inflammatory proteins and transcription factors were assessed by quantitative real time RT-PCR. RESULTS: Individual FAs differentially affect endothelial inflammatory gene expression in a gene-specific manner. EPA, LA and OA significantly up-regulated MCP-1 gene expression compared to AA (p = 0.001, 0.013, 0.008, respectively) and DHA (p < 0.0005, = 0.004, 0.002, respectively). Furthermore, cell stimulation state and FA incubation time significantly influenced reported FA effects on gene expression. CONCLUSION: The comparative effects of saturated, monounsaturated, n-6 and n-3 polyunsaturatedFAs on endothelial gene expression depend on the specific FA investigated, its length of incubation, cell stimulation state and the gene investigated. These findings may explain existing disparity in the literature.
Authors: M A Carluccio; M Massaro; C Bonfrate; L Siculella; M Maffia; G Nicolardi; A Distante; C Storelli Journal: Arterioscler Thromb Vasc Biol Date: 1999-02 Impact factor: 8.311
Authors: Kelly M Boone; Mark A Klebanoff; Lynette K Rogers; Joseph Rausch; Daniel L Coury; Sarah A Keim Journal: Early Hum Dev Date: 2022-05-19 Impact factor: 2.699
Authors: Shu Wang; Dayong Wu; Nirupa R Matthan; Stefania Lamon-Fava; Jaime L Lecker; Alice H Lichtenstein Journal: Atherosclerosis Date: 2008-09-02 Impact factor: 5.162
Authors: Kelly M Boone; Barbara Gracious; Mark A Klebanoff; Lynette K Rogers; Joseph Rausch; Daniel L Coury; Sarah A Keim Journal: Early Hum Dev Date: 2017-09-20 Impact factor: 2.699
Authors: Samantha P Tull; Clara M Yates; Benjamin H Maskrey; Valerie B O'Donnell; Jackie Madden; Robert F Grimble; Philip C Calder; Gerard B Nash; G Ed Rainger Journal: PLoS Biol Date: 2009-08-25 Impact factor: 8.029