AIM: To investigate whether urine is a good medium for screening and whether there is a correlation between the amount of extracted DNA and human papillomavirus (HPV)-positivity. METHODS: In the present study, 30 first-voided urine (FVU) specimens and 20 urethroglandular swabs using cervex-brushes from male partners of HPV-positive patients, and 31 FVU specimens and 100 liquid-based cervix cytology leftovers sampled with cervix-brushes from HPV-positive women were examined for the presence of beta-globin. Oncogenic HPV were detected using type-specific PCR. RESULTS: beta-globin was found in all the brushed samples, whereas it was found in only 68.9% of the FVU specimens. HPV-PCR was positive in 60.0% of the male brushes, in 29% of the female brushes and in 0% of the male FVU specimens. DNA concentration was, respectively, 0.9998 ng/microL, 37.0598 ng/microL and 0.0207 ng/microL. CONCLUSION: Urine is not a good tool for HPV detection, probably because the low DNA concentration reflects a low amount of collected cells. beta-globin is measurable in FVU by real time quantitative PCR, but the DNA concentration is lower compared to brush sampling for both genders. beta-globin-positivity of urethral and cervical swabs is 100%, showing a higher mean concentration of DNA, leading to a higher detection rate of HPV. This is the first article linking DNA-concentration to the presence of HPV.
AIM: To investigate whether urine is a good medium for screening and whether there is a correlation between the amount of extracted DNA and human papillomavirus (HPV)-positivity. METHODS: In the present study, 30 first-voided urine (FVU) specimens and 20 urethroglandular swabs using cervex-brushes from male partners of HPV-positive patients, and 31 FVU specimens and 100 liquid-based cervix cytology leftovers sampled with cervix-brushes from HPV-positive women were examined for the presence of beta-globin. Oncogenic HPV were detected using type-specific PCR. RESULTS:beta-globin was found in all the brushed samples, whereas it was found in only 68.9% of the FVU specimens. HPV-PCR was positive in 60.0% of the male brushes, in 29% of the female brushes and in 0% of the male FVU specimens. DNA concentration was, respectively, 0.9998 ng/microL, 37.0598 ng/microL and 0.0207 ng/microL. CONCLUSION: Urine is not a good tool for HPV detection, probably because the low DNA concentration reflects a low amount of collected cells. beta-globin is measurable in FVU by real time quantitative PCR, but the DNA concentration is lower compared to brush sampling for both genders. beta-globin-positivity of urethral and cervical swabs is 100%, showing a higher mean concentration of DNA, leading to a higher detection rate of HPV. This is the first article linking DNA-concentration to the presence of HPV.
Authors: Chandrika J Piyathilake; Suguna Badiga; Michelle M Chambers; Ilene K Brill; Roland Matthews; Edward E Partridge Journal: Cancer Date: 2016-05-31 Impact factor: 6.860
Authors: K M Kero; J Rautava; K Syrjänen; O Kortekangas-Savolainen; S Grenman; S Syrjänen Journal: Eur J Clin Microbiol Infect Dis Date: 2014-02-07 Impact factor: 3.267
Authors: Vikrant V Sahasrabuddhe; Patti E Gravitt; S Terence Dunn; David Brown; Richard A Allen; Yolanda J Eby; Katie Smith; Rosemary E Zuna; Roy R Zhang; Michael A Gold; Mark Schiffman; Joan L Walker; Philip E Castle; Nicolas Wentzensen Journal: J Clin Microbiol Date: 2013-11-06 Impact factor: 5.948
Authors: Marina Munoz; Milena Camargo; Sara C Soto-De Leon; Ricardo Sanchez; Diana Parra; Andrea C Pineda; Otto Sussmann; Antonio Perez-Prados; Manuel E Patarroyo; Manuel A Patarroyo Journal: PLoS One Date: 2013-02-13 Impact factor: 3.240