Literature DB >> 17703208

Derivation of human embryonic stem cells from single blastomeres.

Irina Klimanskaya1, Young Chung, Sandy Becker, Shi-Jiang Lu, Robert Lanza.   

Abstract

This protocol details a method to derive human embryonic stem (hES) cells from single blastomeres. Blastomeres are removed from morula (eight-cell)-stage embryos and cultured until they form multicell aggregates. These blastomere-derived cell aggregates are plated into microdrops seeded with mitotically inactivated feeder cells, and then connected with neighboring microdrops seeded with green fluorescent protein-positive hES cells. The resulting blastomere-derived outgrowths are cultured in the same manner as blastocyst-derived hES cells. The whole process takes about 3-4 months.

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Year:  2007        PMID： 17703208     DOI： 10.1038/nprot.2007.274

Source DB:  PubMed          Journal:  Nat Protoc        ISSN： 1750-2799            Impact factor:   13.491


  37 in total

Review 1.  Biological characteristics of stem cells from foetal, cord blood and extraembryonic tissues.

Authors:  Hassan Abdulrazzak; Dafni Moschidou; Gemma Jones; Pascale V Guillot
Journal:  J R Soc Interface       Date:  2010-08-25       Impact factor: 4.118

2.  Derivation and propagation of human embryonic stem cell lines from frozen embryos in an animal product-free environment.

Authors:  Emma Stephenson; Laureen Jacquet; Cristian Miere; Victoria Wood; Neli Kadeva; Glenda Cornwell; Stefano Codognotto; Yaser Dajani; Peter Braude; Dusko Ilic
Journal:  Nat Protoc       Date:  2012-06-21       Impact factor: 13.491

Review 3.  Non-embryo-destructive Extraction of Pluripotent Embryonic Stem Cells: Implications for Regenerative Medicine and Reproductive Medicine.

Authors:  R Dittrich; M W Beckmann; W Würfel
Journal:  Geburtshilfe Frauenheilkd       Date:  2015-12       Impact factor: 2.915

4.  Mesenchymal stem cell population derived from human pluripotent stem cells displays potent immunomodulatory and therapeutic properties.

Authors:  Erin A Kimbrel; Nicholas A Kouris; Gregory J Yavanian; Jianlin Chu; Yu Qin; Ann Chan; Ram P Singh; Deborah McCurdy; Lynn Gordon; Ralph D Levinson; Robert Lanza
Journal:  Stem Cells Dev       Date:  2014-05-02       Impact factor: 3.272

Review 5.  Cardiac applications for human pluripotent stem cells.

Authors:  Yuji Shiba; Kip D Hauch; Michael A Laflamme
Journal:  Curr Pharm Des       Date:  2009       Impact factor: 3.116

6.  Establishment, characterization, and differentiation of a karyotypically normal human embryonic stem cell line from a trisomy-affected embryo.

Authors:  Arundhati Mandal; Sheena Mathew; Debapriya Saha; Chandra Viswanathan
Journal:  In Vitro Cell Dev Biol Anim       Date:  2012-12-14       Impact factor: 2.416

7.  Evaluating differentiation propensity of in-house derived human embryonic stem cell lines KIND-1 and KIND-2.

Authors:  Punam Nagvenkar; Prasad Pethe; Harsha Pawani; Jyoti Telang; Neeraj Kumar; Indira Hinduja; Kusum Zaveri; Deepa Bhartiya
Journal:  In Vitro Cell Dev Biol Anim       Date:  2011-05-26       Impact factor: 2.416

8.  Human embryonic stem cells from aneuploid blastocysts identified by pre-implantation genetic screening.

Authors:  Kavita Narwani; Juan-Carlos Biancotti; Tamar Golan-Lev; Nicole Buehler; David Hill; Sagiv Shifman; Nissim Benvenisty; Neta Lavon
Journal:  In Vitro Cell Dev Biol Anim       Date:  2010-03-12       Impact factor: 2.416

Review 9.  Human embryonic stem cells: derivation, maintenance and cryopreservation.

Authors:  Jeoung Eun Lee; Dong Ryul Lee
Journal:  Int J Stem Cells       Date:  2011-06       Impact factor: 2.500

10.  Human embryonic stem cell lines derived from single blastomeres of two 4-cell stage embryos.

Authors:  Mieke Geens; Ileana Mateizel; Karen Sermon; Martine De Rycke; Claudia Spits; Greet Cauffman; Paul Devroey; Herman Tournaye; Inge Liebaers; Hilde Van de Velde
Journal:  Hum Reprod       Date:  2009-07-24       Impact factor: 6.918
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