Literature DB >> 17703067

Describing the structure and assembly of protein filaments by EPR spectroscopy of spin-labeled side chains.

Madhu Budamagunta1, John Hess, Paul Fitzgerald, John Voss.   

Abstract

In this review we summarize our approach to the study of Intermediate Filament (IF) structure and assembly by electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels. Using vimentin, a homopolymeric type III IF protein, we demonstrate that this approach serves as a general paradigm for studying protein filament structure and assembly. These strategies will be useful in exploring the structure and assembly properties of other filamentous or aggregation-prone systems.

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Year:  2007        PMID： 17703067     DOI： 10.1007/s12013-007-0035-4

Source DB:  PubMed          Journal:  Cell Biochem Biophys        ISSN： 1085-9195            Impact factor:   2.194


  2 in total

1.  Site-specific dynamic nuclear polarization of hydration water as a generally applicable approach to monitor protein aggregation.

Authors:  Anna Pavlova; Evan R McCarney; Dylan W Peterson; Frederick W Dahlquist; John Lew; Songi Han
Journal:  Phys Chem Chem Phys       Date:  2009-06-29       Impact factor: 3.676

2.  Identification of phosphorylation-induced changes in vimentin intermediate filaments by site-directed spin labeling and electron paramagnetic resonance.

Authors:  Josh T Pittenger; John F Hess; Madhu S Budamagunta; John C Voss; Paul G Fitzgerald
Journal:  Biochemistry       Date:  2008-09-20       Impact factor: 3.162
  2 in total

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