Literature DB >> 1770199

Cell growth experiments using a microcalorimetric vessel equipped with oxygen and pH electrodes.

P Bäckman1, I Wadsö.   

Abstract

A method is described for simultaneous measurements of heat production rate, oxygen activity and pH, using a 3-ml stirred microcalorimetric titration/perfusion vessel fitted with a polarographic oxygen sensor and a combination pH-electrode. Heat production rate (+/- 0.3 microW), pH (+/- 0.01) and oxygen concentration (+/- 4 mumol/l) could be measured without any cross interference between the three signals. Results from measurements on suspensions of T-lymphoma cells and E. coli are presented.

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Year:  1991        PMID： 1770199     DOI： 10.1016/0165-022x(91)90003-f

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN： 0165-022X


  4 in total

1.  Quantification of vital adherent Streptococcus sanguinis cells on protein-coated titanium after disinfectant treatment.

Authors:  Monika Astasov-Frauenhoffer; Olivier Braissant; Irmgard Hauser-Gerspach; A U Daniels; Dieter Wirz; Roland Weiger; Tuomas Waltimo
Journal:  J Mater Sci Mater Med       Date:  2011-06-14       Impact factor: 3.896

2.  Anaerobic metabolism in the leech (Hirudo medicinalis L.): direct and indirect calorimetry during severe hypoxia.

Authors:  H Schmidt; A Wichmann; I Lamprecht; I Zerbst-Boroffka
Journal:  J Comp Physiol B       Date:  1996       Impact factor: 2.200

3.  Microcalorimetric evaluation of the effects of methotrexate and 6-thioguanine on sensitive T-lymphoma cells and on a methotrexate-resistant subline.

Authors:  J Bermudez; P Bäckman; A Schön
Journal:  Cell Biophys       Date:  1992 Apr-Jun

Review 4.  Living Sample Viability Measurement Methods from Traditional Assays to Nanomotion.

Authors:  Hamzah Al-Madani; Hui Du; Junlie Yao; Hao Peng; Chenyang Yao; Bo Jiang; Aiguo Wu; Fang Yang
Journal:  Biosensors (Basel)       Date:  2022-06-24
  4 in total

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