Literature DB >> 1285295

Microcalorimetric evaluation of the effects of methotrexate and 6-thioguanine on sensitive T-lymphoma cells and on a methotrexate-resistant subline.

J Bermudez1, P Bäckman, A Schön.   

Abstract

Isothermal microcalorimetry was used in order to continuously monitor and quantitatively assess the action of two antineoplastic drugs, methotrexate (MTX) and 6-thioguanine (6-TG), on a human T-lymphoma cell line, CCRF-CEM. The results with MTX were compared with data from experiments with a MTX-resistant subline, CEM/MTX. The slope of the power-time curve after drug injection relative to that obtained during unperturbed growth, was used to construct dose-response curves. The normal cell line was characterized by a D50 value (i.e., the dose producing half the maximal response) of 0.05 microM for MTX and 0.38 microM for 6-TG. For the MTX-resistant subline the D50 value was 8 microM of MTX. Comparisons of the continuous power-time curves showed the inhibitory effect of 6-TG to be faster than that of MTX.

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Year:  1992        PMID： 1285295     DOI： 10.1007/bf02823653

Source DB:  PubMed          Journal:  Cell Biophys        ISSN： 0163-4992


  14 in total

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Authors:  B T Hill; R Baserga
Journal:  Cancer Treat Rev       Date:  1975-09       Impact factor: 12.111

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Authors:  G E FOLEY; H LAZARUS; S FARBER; B G UZMAN; B A BOONE; R E MCCARTHY
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3.  Prediction of the cytotoxic effects of some antineoplastic drugs on cultured T-lymphoma cells by microcalorimetry.

Authors:  T Kimura; A Schön; I Wadsö
Journal:  Cytobios       Date:  1990

4.  Effects of pH-variations on the kinetics of growth and energy metabolism in cultured T-lymphoma cells: a microcalorimetric study.

Authors:  P Bäckman; T Kimura; A Schön; I Wadsö
Journal:  J Cell Physiol       Date:  1992-01       Impact factor: 6.384

5.  Effect of dimethyl sulphoxide and some antibiotics on cultured human T-lymphoma cells as measured by microcalorimetry.

Authors:  P Lönnbro; I Wadsö
Journal:  J Biochem Biophys Methods       Date:  1991 May-Jun

6.  Cell growth experiments using a microcalorimetric vessel equipped with oxygen and pH electrodes.

Authors:  P Bäckman; I Wadsö
Journal:  J Biochem Biophys Methods       Date:  1991-12

7.  Thermochemical characterization of T-lymphoma cells under non-growing conditions.

Authors:  A Schön; I Wadsö
Journal:  Cytobios       Date:  1986

8.  Effects of methotrexate esters and other lipophilic antifolates on methotrexate-resistant human leukemic lymphoblasts.

Authors:  A Rosowsky; H Lazarus; G C Yuan; W R Beltz; L Mangini; H T Abelson; E J Modest; E Frei
Journal:  Biochem Pharmacol       Date:  1980-02-15       Impact factor: 5.858

9.  Design and testing of a new microcalorimetric vessel for use with living cellular systems and in titration experiments.

Authors:  M G Nordmark; J Laynez; A Schön; J Suurkuusk; I Wadsö
Journal:  J Biochem Biophys Methods       Date:  1984-12

10.  The potential use of microcalorimetry in predictive tests of the action of antineoplastic drugs on mammalian cells.

Authors:  A Schön; I Wadsö
Journal:  Cytobios       Date:  1988
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  1 in total

1.  Isothermal microcalorimetry, a new tool to monitor drug action against Trypanosoma brucei and Plasmodium falciparum.

Authors:  Tanja Wenzler; Andrea Steinhuber; Sergio Wittlin; Christian Scheurer; Reto Brun; Andrej Trampuz
Journal:  PLoS Negl Trop Dis       Date:  2012-06-05
  1 in total

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