BACKGROUND AND AIMS: Radiotherapy is frequently used for cancer treatment, but it may be associated with several complications. Thus, this study aimed to evaluate the role of L-glutamine and/or glycine supplementation on the colonic wall in rats submitted to abdominal radiation. MATERIALS AND METHODS: Sixty adult Wistar rats were randomly divided into six groups: I-healthy, II (control)-irradiated rats without amino acid supplementation, III-irradiated rats with glycine supplementation, IV-irradiated rats with L-glutamine supplementation, V-irradiated rats with glycine supplementation 7 days before irradiation and with L-glutamine supplementation 7 days after irradiation, and VI-irradiated rats with L-glutamine supplementation 7 days before irradiation and with glycine supplementation 7 days after irradiation. Abdominal irradiation was employed with a dose of 1,000 cGy on the eighth day of the experiment. All animals underwent laparotomy on the 15th day for resection of a colonic segment for stereologic analysis. Parametric and nonparametric tests were used for statistical analysis, with the level of significance set at p<or=0.05. RESULTS: Control animals (group II) presented a significantly smaller total volume of colonic wall when compared to animals of groups I, III, IV, V, and VI. There was a significant increase in mucosal layer volume in the animals of group IV compared to animals of groups I, II, and III. CONCLUSIONS: Our findings suggest that L-glutamine and/or glycine supplementation is beneficial for the repair of the colonic wall of rats, but L-glutamine, with its trophic effects on the colonic mucosa, seems to exhibit better results.
BACKGROUND AND AIMS: Radiotherapy is frequently used for cancer treatment, but it may be associated with several complications. Thus, this study aimed to evaluate the role of L-glutamine and/or glycine supplementation on the colonic wall in rats submitted to abdominal radiation. MATERIALS AND METHODS: Sixty adult Wistar rats were randomly divided into six groups: I-healthy, II (control)-irradiated rats without amino acid supplementation, III-irradiated rats with glycine supplementation, IV-irradiated rats with L-glutamine supplementation, V-irradiated rats with glycine supplementation 7 days before irradiation and with L-glutamine supplementation 7 days after irradiation, and VI-irradiated rats with L-glutamine supplementation 7 days before irradiation and with glycine supplementation 7 days after irradiation. Abdominal irradiation was employed with a dose of 1,000 cGy on the eighth day of the experiment. All animals underwent laparotomy on the 15th day for resection of a colonic segment for stereologic analysis. Parametric and nonparametric tests were used for statistical analysis, with the level of significance set at p<or=0.05. RESULTS: Control animals (group II) presented a significantly smaller total volume of colonic wall when compared to animals of groups I, III, IV, V, and VI. There was a significant increase in mucosal layer volume in the animals of group IV compared to animals of groups I, II, and III. CONCLUSIONS: Our findings suggest that L-glutamine and/or glycine supplementation is beneficial for the repair of the colonic wall of rats, but L-glutamine, with its trophic effects on the colonic mucosa, seems to exhibit better results.
Authors: F G Campos; D L Waitzberg; D R Mucerino; E L Gonçalves; A F Logulo; A Habr-Gama; J L Rombeau Journal: Nutr Hosp Date: 1996 May-Jun Impact factor: 1.057
Authors: E Olagne; J Baulieux; E de la Roche; M Adham; N Berthoux; O Bourdeix; J P Gerard; C Ducerf Journal: J Am Coll Surg Date: 2000-12 Impact factor: 6.113
Authors: V S Klimberg; W W Souba; D J Dolson; R M Salloum; R D Hautamaki; D A Plumley; W M Mendenhall; F J Bova; S R Khan; R L Hackett Journal: Cancer Date: 1990-07-01 Impact factor: 6.860
Authors: Etiene de Aguiar Picanço; Francisco Lopes-Paulo; Ruy G Marques; Cristina F Diestel; Carlos Eduardo R Caetano; Mônica Vieira Mano de Souza; Gabriela Mendes Moscoso; Helena Maria F Pazos Journal: Int J Colorectal Dis Date: 2011-02-25 Impact factor: 2.571