| Literature DB >> 17687623 |
Claudia E Vickers1, Peer M Schenk, Dongxue Li, Philip M Mullineaux, Peter M Gresshoff.
Abstract
A binary vector containing two reporter gene cassettes has been developed. This vector is ideal for optimising new plant transformation systems. Following optimisation, one of the reporter genes can be replaced with a gene of interest; the second can be used as a marker to confirm transgenic lines, and to estimate locus number and determine zygosity. This allows simple, efficient and economical screening for homozygous single-insert lines and azygous controls.Mesh:
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Year: 2007 PMID: 17687623 DOI: 10.1007/s10529-007-9467-6
Source DB: PubMed Journal: Biotechnol Lett ISSN: 0141-5492 Impact factor: 2.461