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Literature DB >> 17660318

Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters.

Alexander Egner¹, Claudia Geisler, Claas von Middendorff, Hannes Bock, Dirk Wenzel, Rebecca Medda, Martin Andresen, Andre C Stiel, Stefan Jakobs, Christian Eggeling, Andreas Schönle, Stefan W Hell.

Abstract

We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm.

Entities: Chemical Disease Mutation Species

Mesh：

Substances：
Fluorescent Dyes

Year: 2007 PMID： 17660318 PMCID： PMC2025649 DOI： 10.1529/biophysj.107.112201

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

19 in total

1. Ultrahigh-resolution multicolor colocalization of single fluorescent probes.

Authors: T D Lacoste; X Michalet; F Pinaud; D S Chemla; A P Alivisatos; S Weiss
Journal: Proc Natl Acad Sci U S A Date: 2000-08-15 Impact factor: 11.205

2. Precise nanometer localization analysis for individual fluorescent probes.

Authors: Russell E Thompson; Daniel R Larson; Watt W Webb
Journal: Biophys J Date: 2002-05 Impact factor: 4.033

3. 1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants.

Authors: Andre C Stiel; Simon Trowitzsch; Gert Weber; Martin Andresen; Christian Eggeling; Stefan W Hell; Stefan Jakobs; Markus C Wahl
Journal: Biochem J Date: 2007-02-15 Impact factor: 3.857

4. Single-molecule mountains yield nanoscale cell images.

Authors: W E Moerner
Journal: Nat Methods Date: 2006-10 Impact factor: 28.547

5. Proposed method for molecular optical imaging.

Authors: E Betzig
Journal: Opt Lett Date: 1995-02-01 Impact factor: 3.776

Review 6. Improving structural integrity of cryosections for immunogold labeling.

Authors: W Liou; H J Geuze; J W Slot
Journal: Histochem Cell Biol Date: 1996-07 Impact factor: 4.304

7. Visualization of a system of filaments 7-10 nm thick in cultured cells of an epithelioid line (Pt K2) by immunofluorescence microscopy.

Authors: M Osborn; W W Franke; K Weber
Journal: Proc Natl Acad Sci U S A Date: 1977-06 Impact factor: 11.205

8. Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli.

Authors: D E Drew; G von Heijne; P Nordlund; J W de Gier
Journal: FEBS Lett Date: 2001-10-26 Impact factor: 4.124

9. Immunochemistry on ultrathin frozen sections.

Authors: K T Tokuyasu
Journal: Histochem J Date: 1980-07

10. A technique for ultracryotomy of cell suspensions and tissues.

Authors: K T Tokuyasu
Journal: J Cell Biol Date: 1973-05 Impact factor: 10.539

83 in total

1. Optimal 3D single-molecule localization for superresolution microscopy with aberrations and engineered point spread functions.

Authors: Sean Quirin; Sri Rama Prasanna Pavani; Rafael Piestun
Journal: Proc Natl Acad Sci U S A Date: 2011-12-30 Impact factor: 11.205

2. Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density.

Authors: Gregory Giannone; Eric Hosy; Florian Levet; Audrey Constals; Katrin Schulze; Alexander I Sobolevsky; Michael P Rosconi; Eric Gouaux; Robert Tampé; Daniel Choquet; Laurent Cognet
Journal: Biophys J Date: 2010-08-09 Impact factor: 4.033

3. Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength.

Authors: Ilaria Testa; Christian A Wurm; Rebecca Medda; Ellen Rothermel; Claas von Middendorf; Jonas Fölling; Stefan Jakobs; Andreas Schönle; Stefan W Hell; Christian Eggeling
Journal: Biophys J Date: 2010-10-20 Impact factor: 4.033

Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters.

1. Ultrahigh-resolution multicolor colocalization of single fluorescent probes.

2. Precise nanometer localization analysis for individual fluorescent probes.

3. 1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants.

4. Single-molecule mountains yield nanoscale cell images.

5. Proposed method for molecular optical imaging.

Review 6. Improving structural integrity of cryosections for immunogold labeling.

7. Visualization of a system of filaments 7-10 nm thick in cultured cells of an epithelioid line (Pt K2) by immunofluorescence microscopy.

8. Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli.

9. Immunochemistry on ultrathin frozen sections.

10. A technique for ultracryotomy of cell suspensions and tissues.

1. Optimal 3D single-molecule localization for superresolution microscopy with aberrations and engineered point spread functions.

2. Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density.

3. Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength.

4. Combining FISH with localisation microscopy: Super-resolution imaging of nuclear genome nanostructures.

5. Ultrafast, temporally stochastic STED nanoscopy of millisecond dynamics.

6. Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy.

7. Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes.

8. Generation of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy.

Review 9. The fluorescent protein palette: tools for cellular imaging.

10. Photoactivated Localization Microscopy (PALM) of adhesion complexes.