Literature DB >> 17657409

Closed-tube DNA extraction using a thermostable proteinase is highly sensitive, capable of single parasite detection.

B C Ferrari1, M L Power, P L Bergquist.   

Abstract

Current DNA extraction methods for parasites are labour-intensive and usually involve several steps, increasing the potential for cross-contamination. We describe here a closed-tube DNA extraction procedure based upon the use of a thermostable proteinase that enabled sensitive amplification of target loci from parasites from diverse lineages including Apicomplexa, Sarcomastgophora and Nematoda. Moreover, this procedure is not subject to cross-contamination and is readily adaptable to automation.

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Year:  2007        PMID： 17657409     DOI： 10.1007/s10529-007-9487-2

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  7 in total

1.  Evaluation of a PCR protocol for sensitive detection of Giardia intestinalis in human faeces.

Authors:  Amy J Asher; Liette S Waldron; Michelle L Power
Journal:  Parasitol Res       Date:  2011-07-29       Impact factor: 2.289

2.  Molecular epidemiology and spatial distribution of a waterborne cryptosporidiosis outbreak in Australia.

Authors:  Liette S Waldron; Belinda C Ferrari; Cristel Cheung-Kwok-Sang; Paul J Beggs; Nicola Stephens; Michelle L Power
Journal:  Appl Environ Microbiol       Date:  2011-09-09       Impact factor: 4.792

3.  Molecular epidemiology, spatiotemporal analysis, and ecology of sporadic human cryptosporidiosis in Australia.

Authors:  Liette S Waldron; Borce Dimeski; Paul J Beggs; Belinda C Ferrari; Michelle L Power
Journal:  Appl Environ Microbiol       Date:  2011-09-09       Impact factor: 4.792

4.  Terminal restriction fragment length polymorphism for identification of Cryptosporidium species in human feces.

Authors:  L S Waldron; B C Ferrari; M R Gillings; M L Power
Journal:  Appl Environ Microbiol       Date:  2008-10-31       Impact factor: 4.792

5.  Recovering greater fungal diversity from pristine and diesel fuel contaminated sub-antarctic soil through cultivation using both a high and a low nutrient media approach.

Authors:  Belinda C Ferrari; Chengdong Zhang; Josie van Dorst
Journal:  Front Microbiol       Date:  2011-11-15       Impact factor: 5.640

6.  Direct cell lysis for single-cell gene expression profiling.

Authors:  David Svec; Daniel Andersson; Milos Pekny; Robert Sjöback; Mikael Kubista; Anders Ståhlberg
Journal:  Front Oncol       Date:  2013-11-07       Impact factor: 6.244

7.  Insights into the distribution and abundance of the ubiquitous candidatus Saccharibacteria phylum following tag pyrosequencing.

Authors:  Belinda Ferrari; Tristrom Winsley; Mukan Ji; Brett Neilan
Journal:  Sci Rep       Date:  2014-02-04       Impact factor: 4.379
  7 in total

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