Literature DB >> 1765655

An isoelectric focusing method for the study of the humoral response against the antigen 85 complex of Mycobacterium bovis BCG in the different forms of leprosy.

A Drowart1, P Launois, M De Cock, K Huygen, J De Bruyn, J C Yernault, J P Van Vooren.   

Abstract

Isoelectric focusing was used to separate the three components of the antigen 85 complex of Mycobacterium bovis BCG. Antibody responses of leprosy patients against each Mycobacterium bovis BCG. Antibody responses of leprosy patients against each component were quantitated by densitometric analysis of immunoblot assays. The 85A component was recognized by 40% (8/20) of the lepromin positive and negative healthy subjects, by 76% (19/25) of the tuberculoid and by 96% (24/25) of the lepromatous leprosy sera. In contrast, the 85B component was not stained by the control sera, nor by the tuberculoid leprosy sera but by 64% (16/25) of the lepromatous leprosy sera. The results suggest that antigen 85B contains one or several epitopes that are specifically recognized by sera of lepromatous leprosy patients only.

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Year:  1991        PMID： 1765655     DOI： 10.1016/0022-1759(91)90330-i

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  8 in total

1.  Humoral responses against the 85A and 85B antigens of Mycobacterium bovis BCG in patients with leprosy and tuberculosis.

Authors:  J P Van Vooren; A Drowart; J De Bruyn; P Launois; J Millan; E Delaporte; M Develoux; J C Yernault; K Huygen
Journal:  J Clin Microbiol       Date:  1992-06       Impact factor: 5.948

2.  Purification of Mycobacterium bovis BCG Tokyo antigens by chromatofocusing, lectin-affinity chromatography, and hydrophobic interaction chromatography.

Authors:  E A Sugden; K Stilwell; D C Watson; E B Rohonczy; P Martineau
Journal:  Clin Diagn Lab Immunol       Date:  1996-09

3.  Enoyl-coenzyme A hydratase and antigen 85B of Mycobacterium habana are specifically recognized by antibodies in sera from leprosy patients.

Authors:  J Serafín-López; M Talavera-Paulin; J C Amador-Molina; M Alvarado-Riverón; M M Vilchis-Landeros; P Méndez-Ortega; M Fafutis-Morris; V Paredes-Cervantes; R López-Santiago; C I León; M I Guerrero; R M Ribas-Aparicio; G Mendoza-Hernández; C Carreño-Martínez; S Estrada-Parra; I Estrada-García
Journal:  Clin Vaccine Immunol       Date:  2011-05-25

4.  T cell reactivity against antigen 85 but not against the 18- and 65-kD heat shock proteins in the early stages of acquired immunity against Mycobacterium leprae.

Authors:  P Launois; M Niang N'Diaye; J L Sarthou; A Drowart; J P Van Vooren; J L Cartel; K Huygen
Journal:  Clin Exp Immunol       Date:  1994-04       Impact factor: 4.330

5.  The Mycobacterium leprae antigen 85 complex gene family: identification of the genes for the 85A, 85C, and related MPT51 proteins.

Authors:  T F Rinke de Wit; S Bekelie; A Osland; B Wieles; A A Janson; J E Thole
Journal:  Infect Immun       Date:  1993-09       Impact factor: 3.441

6.  Mapping of murine Th1 helper T-Cell epitopes of mycolyl transferases Ag85A, Ag85B, and Ag85C from Mycobacterium tuberculosis.

Authors:  S D'Souza; V Rosseels; M Romano; A Tanghe; O Denis; F Jurion; N Castiglione; A Vanonckelen; K Palfliet; Kris Huygen
Journal:  Infect Immun       Date:  2003-01       Impact factor: 3.441

7.  Mycobacterium leprae produces extracellular homologs of the antigen 85 complex.

Authors:  M C Pessolani; P J Brennan
Journal:  Infect Immun       Date:  1992-11       Impact factor: 3.441

8.  T-cell-epitope mapping of the major secreted mycobacterial antigen Ag85A in tuberculosis and leprosy.

Authors:  P Launois; R DeLeys; M N Niang; A Drowart; M Andrien; P Dierckx; J L Cartel; J L Sarthou; J P Van Vooren; K Huygen
Journal:  Infect Immun       Date:  1994-09       Impact factor: 3.441
  8 in total

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