A Soluble Precipitating Antigen From Hog Cholera Virus Propagated in Tissue Culture: I. Preparation and Characterization of the Antigen.

A precipitating hog cholera viral antigen was produced in a swine kidney tissue culture system. This antigen produced a single, sharp precipitin line with reference antiserum but no precipitin lines with nonimmune control sera. The precipitating antigen was present in the supernatant fluid after high-speed centrifugation, and was readily separated from infective virus by diethylaminoethyl cellulose (DEAE) chromatography. The antigen was found to be nondialyzable, stable to the action of chloroform and to storage at refrigeration temperature for at least 7 mo, but was inactivated at 56 C in 30 min.