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Literature DB >> 17649467

Identification of Hog Cholera Viral Antigen by Immunofluorescence. Application as a Diagnostic and Assay Method.

Abstract

In the absence of detectable cytologic changes in hog cholera virus-infected tissue culture cells, hog cholera viral antigen was readily detected by immunofluorescence. The ability to detect hog cholera viral antigen by this method allowed for determination of infectivity titers and also for titration of homologous antibody. Immunofluorescence made possible the identification, in tissue culture, of hog cholera virus from blood, serum, and spleen extracts of experimentally infected swine. Further applications of this method and its limitations are being investigated.

Entities: Species

Year: 1963 PMID： 17649467 PMCID： PMC1583710

Source DB: PubMed Journal: Can J Comp Med Vet Sci ISSN： 0316-5957

5 in total

1. Relationship between hog cholera virus and virus diarrhea virus of cattle.

Authors: B E SHEFFY; L COGGINS; J A BAKER
Journal: Proc Soc Exp Biol Med Date: 1962-02

2. Propagation of hog cholera virus in tissue culture.

Authors: J H GILLESPIE; B E SHEFFY; J A BAKER
Journal: Proc Soc Exp Biol Med Date: 1960-12

3. Variations (variants) of hog cholera virus.

Authors: C N DALE; H W SCHOENING; C G COLE; R R HENLEY; M R ZINOBER
Journal: J Am Vet Med Assoc Date: 1951-05 Impact factor: 1.936

4. Separation of a Soluble Antigen and Infectious Particles of Bovine Viral Diarrhea Viruses and their Relationship to Hog Cholera.

Authors: D E Gutekunst; W A Malmquist
Journal: Can J Comp Med Vet Sci Date: 1963-05

5. Demonstration of an Antigenic Relationship Between Hog Cholera and Bovine Viral Diarrhea Viruses by Immunofluorescence.

Authors: W L Mengeling; D E Gutekunst; A L Fernelius; E C Pirtle
Journal: Can J Comp Med Vet Sci Date: 1963-07

5 in total

15 in total

1. Characterization of bovine viral diarrhea viruses. IV. Sequential development of BVD viral antigen in cell cultures studied by immunofluorescence.

Authors: A L Fernelius
Journal: Arch Gesamte Virusforsch Date: 1969

2. A fluorescent micro-plaque assay for hog cholera virus. Localization of infection with homologous antiserum.

Authors: W L Mengeling
Journal: Arch Gesamte Virusforsch Date: 1968

3. Detection of antibodies against hog cholera virus and bovine viral diarrhea virus in porcine serum. A comparative examination using CF, PLA and NPLA assays.

Authors: M H Jensen
Journal: Acta Vet Scand Date: 1981 Impact factor: 1.695

4. The effects of in utero viral infection on embryonic, fetal, and neonatal survival: a comparison of SMEDI (porcine picorna) viruses with hog cholera vaccinal virus.

Authors: H W Dunne; J T Wang; C D Clark; J F Hokanson; T Morimoto; G R Bubash
Journal: Can J Comp Med Date: 1969-10

8. A Soluble Precipitating Antigen From Hog Cholera Virus Propagated in Tissue Culture: I. Preparation and Characterization of the Antigen.

Authors: E C Pirtle
Journal: Can J Comp Med Vet Sci Date: 1964-08

9. Hog Cholera: I. Investigation of the Agar Double-Diffusion Precipitation Test for The Detection of the Virus in Swine Tissue.

Authors: G M Ruckerbauer; M Appel; G L Bannister; K Mori; D Cochrane; P Boulanger
Journal: Can J Comp Med Vet Sci Date: 1964-12

10. Noncytopathogenic Bovine Viral Diarrhea Viruses Detected and Titrated by Immunofluorescence.

Authors: A L Fernelius
Journal: Can J Comp Med Vet Sci Date: 1964-05