PURPOSE: This study was designed to examine the effect of bacterial contamination on in vitro fertilization treatment outcomes. METHOD: In a prospective clinical trial, 152 patients aged 23-38 years, mean 33.3 +/- 4.6, undergoing IVF treatment were selected for this study. During embryo transfer, separate samples were collected for microbial examination from the following sites: the fundus of the vagina, the cervix, the embryo culture medium prior and post-embryo transfer, the tip of the catheter, and the external sheet. All the samples were separately cultured to identify any bacteria or yeast present. RESULTS: Pregnancy rates in patients testing positive for Entrobacteriaceae (22.2% versus 51%) and Staphylococcus species (17.6% versus 44%) were significantly lower than those in the negative culture group (p < 0.001). The pregnancy rates do not seem to be affected by the other isolated microorganisms. CONCLUSION: This study shows that the presence of vaginal-cervical microbial contamination at the time of embryo transfer is associated with significantly decreased pregnancy rates.
PURPOSE: This study was designed to examine the effect of bacterial contamination on in vitro fertilization treatment outcomes. METHOD: In a prospective clinical trial, 152 patients aged 23-38 years, mean 33.3 +/- 4.6, undergoing IVF treatment were selected for this study. During embryo transfer, separate samples were collected for microbial examination from the following sites: the fundus of the vagina, the cervix, the embryo culture medium prior and post-embryo transfer, the tip of the catheter, and the external sheet. All the samples were separately cultured to identify any bacteria or yeast present. RESULTS: Pregnancy rates in patients testing positive for Entrobacteriaceae (22.2% versus 51%) and Staphylococcus species (17.6% versus 44%) were significantly lower than those in the negative culture group (p < 0.001). The pregnancy rates do not seem to be affected by the other isolated microorganisms. CONCLUSION: This study shows that the presence of vaginal-cervical microbial contamination at the time of embryo transfer is associated with significantly decreased pregnancy rates.
Authors: Richard W Hyman; Christopher N Herndon; Hui Jiang; Curtis Palm; Marilyn Fukushima; Denise Bernstein; Kim Chi Vo; Zara Zelenko; Ronald W Davis; Linda C Giudice Journal: J Assist Reprod Genet Date: 2012-01-06 Impact factor: 3.412
Authors: J M Franasiak; M D Werner; C R Juneau; X Tao; J Landis; Y Zhan; N R Treff; R T Scott Journal: J Assist Reprod Genet Date: 2015-11-07 Impact factor: 3.412
Authors: Hilary O D Critchley; Elnur Babayev; Serdar E Bulun; Sandy Clark; Iolanda Garcia-Grau; Peter K Gregersen; Aoife Kilcoyne; Ji-Yong Julie Kim; Missy Lavender; Erica E Marsh; Kristen A Matteson; Jacqueline A Maybin; Christine N Metz; Inmaculada Moreno; Kami Silk; Marni Sommer; Carlos Simon; Ridhi Tariyal; Hugh S Taylor; Günter P Wagner; Linda G Griffith Journal: Am J Obstet Gynecol Date: 2020-07-21 Impact factor: 10.693