Literature DB >> 17634598

Methods for high-throughput materialization of genetic information based on wheat germ cell-free expression system.

Tatsuya Sawasaki1, Ryo Morishita, Mudeppa D Gouda, Yaeta Endo.   

Abstract

Among the cell-free protein synthesis systems, the wheat germ-based translation system has significant advantages for the high-throughput production of eukaryotic multidomain proteins in folded state. Here, we describe protocols for this cell-free expression system.

Entities:  

Mesh：

Substances：

Year:  2007        PMID： 17634598     DOI： 10.1007/978-1-59745-388-2_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  17 in total

1.  Plant Aurora kinases interact with and phosphorylate transcription factors.

Authors:  Mai Takagi; Takuya Sakamoto; Ritsuko Suzuki; Keiichirou Nemoto; Takeshi Obayashi; Takeshi Hirakawa; Tomoko M Matsunaga; Daisuke Kurihara; Yuko Nariai; Takeshi Urano; Tatsuya Sawasaki; Sachihiro Matsunaga
Journal:  J Plant Res       Date:  2016-10-12       Impact factor: 2.629

2.  Overview of cell-free protein synthesis: historic landmarks, commercial systems, and expanding applications.

Authors:  Shaorong Chong
Journal:  Curr Protoc Mol Biol       Date:  2014-10-01

3.  Identification of target proteins of clinical immunity to Plasmodium falciparum in a region of low malaria transmission.

Authors:  Hirokazu Sakamoto; Satoru Takeo; Eizo Takashima; Kazutoyo Miura; Bernard N Kanoi; Takamasa Kaneko; Eun-Taek Han; Mayumi Tachibana; Kazuhiro Matsuoka; Jetsumon Sattabongkot; Rachanee Udomsangpetch; Tomoko Ishino; Takafumi Tsuboi
Journal:  Parasitol Int       Date:  2017-12-05       Impact factor: 2.230

4.  Simple screening method for autoantigen proteins using the N-terminal biotinylated protein library produced by wheat cell-free synthesis.

Authors:  Kazuhiro Matsuoka; Hiroaki Komori; Masato Nose; Yaeta Endo; Tatsuya Sawasaki
Journal:  J Proteome Res       Date:  2010-08-06       Impact factor: 4.466

5.  Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins.

Authors:  Satoko Matsunaga; Kazuhiro Matsuoka; Kouhei Shimizu; Yaeta Endo; Tatsuya Sawasaki
Journal:  BMC Biotechnol       Date:  2010-06-04       Impact factor: 2.563

6.  Species-independent translational leaders facilitate cell-free expression.

Authors:  Sergei Mureev; Oleksiy Kovtun; Uyen T T Nguyen; Kirill Alexandrov
Journal:  Nat Biotechnol       Date:  2009-08-02       Impact factor: 54.908

7.  Biallelic Mutations in Nuclear Pore Complex Subunit NUP107 Cause Early-Childhood-Onset Steroid-Resistant Nephrotic Syndrome.

Authors:  Noriko Miyake; Hiroyasu Tsukaguchi; Eriko Koshimizu; Akemi Shono; Satoko Matsunaga; Masaaki Shiina; Yasuhiro Mimura; Shintaro Imamura; Tomonori Hirose; Koji Okudela; Kandai Nozu; Yuko Akioka; Motoshi Hattori; Norishige Yoshikawa; Akiko Kitamura; Hae Il Cheong; Shoji Kagami; Michiaki Yamashita; Atsushi Fujita; Satoko Miyatake; Yoshinori Tsurusaki; Mitsuko Nakashima; Hirotomo Saitsu; Kenichi Ohashi; Naoko Imamoto; Akihide Ryo; Kazuhiro Ogata; Kazumoto Iijima; Naomichi Matsumoto
Journal:  Am J Hum Genet       Date:  2015-09-24       Impact factor: 11.025

Review 8.  Engine out of the chassis: cell-free protein synthesis and its uses.

Authors:  Gabriel Rosenblum; Barry S Cooperman
Journal:  FEBS Lett       Date:  2013-10-22       Impact factor: 4.124

9.  Characterization of a caspase-3-substrate kinome using an N- and C-terminally tagged protein kinase library produced by a cell-free system.

Authors:  D Tadokoro; S Takahama; K Shimizu; S Hayashi; Y Endo; T Sawasaki
Journal:  Cell Death Dis       Date:  2010-10-28       Impact factor: 8.469

10.  The phosphorylation of HIV-1 Gag by atypical protein kinase C facilitates viral infectivity by promoting Vpr incorporation into virions.

Authors:  Ayumi Kudoh; Shoukichi Takahama; Tatsuya Sawasaki; Hirotaka Ode; Masaru Yokoyama; Akiko Okayama; Akiyo Ishikawa; Kei Miyakawa; Satoko Matsunaga; Hirokazu Kimura; Wataru Sugiura; Hironori Sato; Hisashi Hirano; Shigeo Ohno; Naoki Yamamoto; Akihide Ryo
Journal:  Retrovirology       Date:  2014-01-22       Impact factor: 4.602
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.