| Literature DB >> 17634595 |
Vladimir A Shirokov1, Aigar Kommer, Vyacheslav A Kolb, Alexander S Spirin.
Abstract
Protein synthesis in cell-free systems is an emerging technology already competing with in vivo expression methods. In this chapter the basic principles of continuous-exchange protein synthesizing systems, and protocols for Escherichia coli and wheat germ translation and transcription-translation systems are described. The ways to improve substrate supply in cell-free systems and mRNA design for eukaryotic system are discussed. Correct folding of the synthesized protein is demonstrated and discussed in detail.Entities:
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Year: 2007 PMID： 17634595 DOI： 10.1007/978-1-59745-388-2_2
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745