| Literature DB >> 17632078 |
Akihiro Tomoyasu1, Kanji Higashio, Kazuhiro Kanomata, Masaaki Goto, Kunihiko Kodaira, Hiroko Serizawa, Tatsuo Suda, Atsushi Nakamura, Junya Nojima, Toru Fukuda, Takenobu Katagiri.
Abstract
Platelet-rich plasma (PRP) is clinically used as an autologous blood product to stimulate bone formation in vivo. In the present study, we examined the effects of PRP on proliferation and osteoblast differentiation in vitro in the presence of bone morphogenetic proteins (BMPs). PRP and its soluble fraction stimulated osteoblastic differentiation of myoblasts and osteoblastic cells in the presence of BMP-2, BMP-4, BMP-6 or BMP-7. The soluble PRP fraction stimulated osteoblastic differentiation in 3D cultures using scaffolds made of collagen or hydroxyapatite. Moreover, heparin-binding fractions obtained from serum also stimulated osteoblastic differentiation in the presence of BMP-4. These results suggested that platelets contain not only growth factors for proliferation but also novel potentiator(s) for BMP-dependent osteoblastic differentiation.Entities:
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Year: 2007 PMID： 17632078 DOI： 10.1016/j.bbrc.2007.06.142
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575