Bogdan-CĂtĂlin Alexandru1, Monica Popa1, Liviu Oana2, Cosmina Ioana Bondor3, Carmen Georgiu4, Camelia-Sidonia LazĂr5, Alina Simona Șovrea6, Anne-Marie Constantin6, Gabriela Dogaru7. 1. Discipline of Medical Hygiene, "Iuliu Hațieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania. 2. Department of Surgical Pathology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania. 3. Department of Medical Informatics and Biostatistics, "Iuliu Hațieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania. 4. Discipline of Pathology, Department of Morphological Sciences, "Iuliu Hațieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania; carmengeorgiu@hotmail.com. 5. Discipline of Pathology, Department of Morphological Sciences, "Iuliu Hațieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania. 6. Discipline of Histology, Department of Morphological Sciences, "Iuliu Hațieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania. 7. Discipline of Medical Rehabilitation, "Iuliu Hațieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Abstract
BACKGROUND/AIM: The biomaterials used in guided bone regeneration have undergone significant diversification in recent years. This study aimed to evaluate alveolar bone addition and bone morphogenetic protein 7 (BMP7) expression using an improved autologous and xenogeneic biomaterial. MATERIALS AND METHODS: Chronic marginal periodontitis was induced in sheep; the intervention group received bone addition as periodontal therapy, using a composite system with lyophilized bovine bone enriched with atelocollagen type 1, platelet-rich plasma and advanced platelet-rich fibrin (A-PRF). Six weeks after the intervention, the dentoalveolar structures were evaluated using hematoxylin-eosin and immunohistochemical staining, to evaluate bone addition and BMP7 expression. RESULTS: The untreated sheep showed inflammation, periodontal ligament destruction, remnants of calculus and bacterial plaque as well as foreign bodies in the desmodontal space, without sings of repair. In the treated sheep, fibroblasts/fibrosis, cartilage and/or new bone, cellular cementum and desmodontium, along with remnants of biomaterial with various degrees of cellularity were observed. In the untreated group, the presence of BMP7 was found in osteoblasts and osteocytes while in the treated group, it was mainly found in the biomaterial remnants, while immunohistochemical staining was less intense in the newly formed osteo-periodontal tissues. Quantitative analysis using the Mann-Whitney U-test showed highly statistically significant differences between the two groups, demonstrating the efficiency of this composite system. CONCLUSION: The current composite system meets all the necessary conditions for promising guided alveolar bone regeneration. Copyright
BACKGROUND/AIM: The biomaterials used in guided bone regeneration have undergone significant diversification in recent years. This study aimed to evaluate alveolar bone addition and bone morphogenetic protein 7 (BMP7) expression using an improved autologous and xenogeneic biomaterial. MATERIALS AND METHODS: Chronic marginal periodontitis was induced in sheep; the intervention group received bone addition as periodontal therapy, using a composite system with lyophilized bovine bone enriched with atelocollagen type 1, platelet-rich plasma and advanced platelet-rich fibrin (A-PRF). Six weeks after the intervention, the dentoalveolar structures were evaluated using hematoxylin-eosin and immunohistochemical staining, to evaluate bone addition and BMP7 expression. RESULTS: The untreated sheep showed inflammation, periodontal ligament destruction, remnants of calculus and bacterial plaque as well as foreign bodies in the desmodontal space, without sings of repair. In the treated sheep, fibroblasts/fibrosis, cartilage and/or new bone, cellular cementum and desmodontium, along with remnants of biomaterial with various degrees of cellularity were observed. In the untreated group, the presence of BMP7 was found in osteoblasts and osteocytes while in the treated group, it was mainly found in the biomaterial remnants, while immunohistochemical staining was less intense in the newly formed osteo-periodontal tissues. Quantitative analysis using the Mann-Whitney U-test showed highly statistically significant differences between the two groups, demonstrating the efficiency of this composite system. CONCLUSION: The current composite system meets all the necessary conditions for promising guided alveolar bone regeneration. Copyright
Authors: Angad Malhotra; Matthew Pelletier; Rema Oliver; Chris Christou; William R Walsh Journal: Tissue Eng Part A Date: 2014-04-28 Impact factor: 3.845