| Literature DB >> 17629372 |
Mahmoud R Jaafari1, Ali Badiee, Ali Khamesipour, Afshin Samiei, Dina Soroush, Masoumeh Tavassoti Kheiri, Farzaneh Barkhordari, W Robert McMaster, Fereidoun Mahboudi.
Abstract
CpG oligodeoxynucleotides (CpG ODN) are known to be a potent immunoadjuvant for a wide range of antigens. The aim of this study was to evaluate the role of CpG ODN co-encapsulated with rgp63 antigen in cationic liposomes (Lip-rgp63-CpG ODN) in immune response enhancement and protection in BALB/c mice against leishmaniasis. Lip-rgp63-CpG ODN prepared by using dehydration-rehydration vesicle (DRV) method significantly inhibited (P<0.001) Leishmania major infection in mice measured by footpad swelling compared to Lip-rgp63, rgp63 alone, rgp63 plus CpG ODN, PBS or control liposomes. The mice immunized with Lip-rgp63-CpG ODN also showed the lowest spleen parasite burden, highest IgG2a/IgG1 ratio and IFN-gamma production and the lowest IL-4 production compared to the other groups. The results indicate that co-encapsulation of CpG ODN in liposomes improves the immunogenicity of Leishmania antigen.Entities:
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Year: 2007 PMID： 17629372 DOI： 10.1016/j.vaccine.2007.05.009
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641