Literature DB >> 1762912

Requirement of protein factors and ATP for the disassembly of the spliceosome after mRNA splicing reaction.

H Sawa1, Y Shimura.   

Abstract

Pre-mRNA splicing reaction occurs in a large ribonucleoprotein complex called the spliceosome. After the splicing reaction, the spliceosome is disassembled to release the splicing products including spliced mRNA. Here we show that protein factors in a HeLa nuclear extract or a DEAE-cellulose fraction as well as ATP are required to release the splicing products form the spliceosome in which the splicing reaction has already completed.

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Year:  1991        PMID： 1762912      PMCID： PMC329315          DOI： 10.1093/nar/19.24.6819

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  10 in total

1.  Alterations of RNase H sensitivity of the 3' splice site region during the in vitro splicing reaction.

Authors:  H Sawa; Y Shimura
Journal:  Nucleic Acids Res       Date:  1991-07-25       Impact factor: 16.971

2.  Requirement of the RNA helicase-like protein PRP22 for release of messenger RNA from spliceosomes.

Authors:  M Company; J Arenas; J Abelson
Journal:  Nature       Date:  1991-02-07       Impact factor: 49.962

3.  An RNA processing activity that debranches RNA lariats.

Authors:  B Ruskin; M R Green
Journal:  Science       Date:  1985-07-12       Impact factor: 47.728

4.  RNA splicing products formed with isolated fractions from HeLa cells are associated with fast-sedimenting complexes.

Authors:  K K Perkins; H M Furneaux; J Hurwitz
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

5.  Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes.

Authors:  M M Konarska; P A Sharp
Journal:  Cell       Date:  1987-06-19       Impact factor: 41.582

6.  Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs.

Authors:  M M Konarska; P A Sharp
Journal:  Cell       Date:  1986-09-12       Impact factor: 41.582

Review 7.  Pre-mRNA splicing.

Authors:  M R Green
Journal:  Annu Rev Genet       Date:  1986       Impact factor: 16.830

8.  Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

Authors:  J D Dignam; R M Lebovitz; R G Roeder
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971

9.  Isolation and characterization of the gene encoding yeast debranching enzyme.

Authors:  K B Chapman; J D Boeke
Journal:  Cell       Date:  1991-05-03       Impact factor: 41.582

10.  Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for pre-mRNA splicing in vitro.

Authors:  A R Krainer; T Maniatis
Journal:  Cell       Date:  1985-10       Impact factor: 41.582
  10 in total
  3 in total

1.  Prp22, a DExH-box RNA helicase, plays two distinct roles in yeast pre-mRNA splicing.

Authors:  B Schwer; C H Gross
Journal:  EMBO J       Date:  1998-04-01       Impact factor: 11.598

Review 2.  Splicing of precursors to mRNA in higher plants: mechanism, regulation and sub-nuclear organisation of the spliceosomal machinery.

Authors:  G G Simpson; W Filipowicz
Journal:  Plant Mol Biol       Date:  1996-10       Impact factor: 4.076

3.  A single SR-like protein, Npl3, promotes pre-mRNA splicing in budding yeast.

Authors:  Tracy L Kress; Nevan J Krogan; Christine Guthrie
Journal:  Mol Cell       Date:  2008-12-05       Impact factor: 17.970
  3 in total

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