OBJECTIVE: To investigate the immunocytochemical and mRNA expression of bone morphogenetic proteins 4 (BMP-4) and 7 (BMP-7) and their three receptors (BMPR-IA, BMPR-IB, and BMPR-II) in ovaries from human adults and fetuses. DESIGN: Immunocytochemical and in situ hybridization study. SETTING: Major tertiary care and referral academic centers. PATIENT(S): Sixteen adolescents/adults aged 13-38 years and 31 women undergoing second and third trimester pregnancy terminations. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Immunocytochemistry and in situ hybridization on paraffin sections of human ovaries from fetuses and adults. RESULT(S): The expression of the proteins for BMP-4 and BMP-7 and their receptors was detected in oogonia/oocytes and stroma cells from both sources (fetuses and women/adolescents). BMP-7 and the three receptors were identified in all of these granulosa cells, and BMP-4 was detected only in the granulosa cells of women/adolescents. Transcripts for all five ligands were identified in stroma cells of all samples and in fetal oogonia/oocytes. BMPR-IA and BMPR-IB mRNA expression was also identified in oocytes from women/adolescents. BMP-7 and BMPR-IA mRNA staining was detected in fetal granulosa cells. CONCLUSION(S): This is the first report of the expression of BMP-4 and BMP-7 and their receptors in human ovaries from fetuses as well as adults. However, to elucidate whether indeed BMP-4 or BMP-7 are involved in the initiation of human primordial follicular growth, they should be added to the culture medium.
OBJECTIVE: To investigate the immunocytochemical and mRNA expression of bone morphogenetic proteins 4 (BMP-4) and 7 (BMP-7) and their three receptors (BMPR-IA, BMPR-IB, and BMPR-II) in ovaries from human adults and fetuses. DESIGN: Immunocytochemical and in situ hybridization study. SETTING: Major tertiary care and referral academic centers. PATIENT(S): Sixteen adolescents/adults aged 13-38 years and 31 women undergoing second and third trimester pregnancy terminations. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Immunocytochemistry and in situ hybridization on paraffin sections of human ovaries from fetuses and adults. RESULT(S): The expression of the proteins for BMP-4 and BMP-7 and their receptors was detected in oogonia/oocytes and stroma cells from both sources (fetuses and women/adolescents). BMP-7 and the three receptors were identified in all of these granulosa cells, and BMP-4 was detected only in the granulosa cells of women/adolescents. Transcripts for all five ligands were identified in stroma cells of all samples and in fetal oogonia/oocytes. BMPR-IA and BMPR-IB mRNA expression was also identified in oocytes from women/adolescents. BMP-7 and BMPR-IA mRNA staining was detected in fetal granulosa cells. CONCLUSION(S): This is the first report of the expression of BMP-4 and BMP-7 and their receptors in human ovaries from fetuses as well as adults. However, to elucidate whether indeed BMP-4 or BMP-7 are involved in the initiation of human primordial follicular growth, they should be added to the culture medium.
Authors: Luciana Ochuiuto Teixeira de Resende; Alessandra Aparecida Vireque; Laura Ferreira Santana; Daniel Antunes Moreno; Ana Carolina Japur de Sá Rosa e Silva; Rui Alberto Ferriani; Carlos Alberto Scrideli; Rosana Maria Reis Journal: J Assist Reprod Genet Date: 2012-07-24 Impact factor: 3.412
Authors: Andrew J Childs; Hazel L Kinnell; Craig S Collins; Kirsten Hogg; Rosemary A L Bayne; Samira J Green; Alan S McNeilly; Richard A Anderson Journal: Stem Cells Date: 2010-08 Impact factor: 6.277
Authors: A B Brito; R R Santos; R van den Hurk; J S Lima; M S Miranda; O M Ohashi; S F S Domingues Journal: Reprod Sci Date: 2013-01-11 Impact factor: 3.060
Authors: Arivaldo José Conceição Meireles; João Paolo Bilibio; Pânila Longhi Lorenzzoni; Emily De Conto; Fábio Costa do Nascimento; João Sabino da Cunha-Filho Journal: JBRA Assist Reprod Date: 2021-07-21