| Literature DB >> 176152 |
S Wang, J F Chiu, L Klyszejko-Stefanowicz, H Fujitani, L S Hnilica.
Abstract
A group of chromosomal non-histone proteins with affinity for DNA (NP) was isolated from rat liver and Novikoff hepatoma. This fraction, which represents less than 5% of the total chromatin protein content, binds preferentially to unique, double-stranded sequences of fractionated homologous DNA. The interactions are strong at low ionic strength (Km = 6.7 X 10(-9) M) and decrease with rising salt concentration. Complexes of the NP protein fraction with homologous DNA are immunologically tissue-specific. As determined by microcomplement fixation, the NP proteins in Novikoff hepatoma are associated with the transcriptionally active, diffuse fraction of chromatin.Entities:
Mesh:
Substances:
Year: 1976 PMID: 176152
Source DB: PubMed Journal: J Biol Chem ISSN: 0021-9258 Impact factor: 5.157