| Literature DB >> 17601805 |
Yohann Couté1, Céline Hernandez, Ron D Appel, Jean-Charles Sanchez, Abelardo Margolles.
Abstract
Stable isotope labeling of amino acids in cell culture was used for Bifidobacterium longum. A comprehensive proteomic strategy was developed and validated by designing an appropriate semidefined medium that allows stable replacement of natural leucine by [(13)C6]leucine. Using this strategy, proteins having variations of at least 50% in their expression rates can be quantified with great confidence.Entities:
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Year: 2007 PMID: 17601805 PMCID: PMC2042066 DOI: 10.1128/AEM.00667-07
Source DB: PubMed Journal: Appl Environ Microbiol ISSN: 0099-2240 Impact factor: 4.792