AIM: To investigate the expression of PCNA, Ki-67 and p53 antibodies in colorectal carcinomas and to establish the relationship between these markers and some particular histological findings of colorectal carcinomas. MATERIAL AND METHODS: We determined immunohistochemically the expression of PCNA, Ki-67 and p53 antibodies in 41 cases of colorectal carcinomas. RESULTS: In adenocarcinomas, the tumor proliferative activity, detected with PCNA and Ki-67 antibodies, increased with the histological grade. Mucinous adenocarcinomas had a mean PCNA LI of 50% and a mean Ki-67 LI of 32%, while signet ring carcinomas had a mean PCNA LI of 70% and a mean Ki-67 LI of 45%. The proliferative activity in the foci of squamous metaplasia was lower than the proliferative activity of malignant areas in the analyzed adenocarcinomas. The p53 overexpression was detected in 24 cases (58.53%). In adenocarcinomas, the p53 positive rate increased with the dedifferentiation of these tumours. Only 16.66% of the cases of carcinomas with mucus secreting cells overexpressed p53, while adenocarcinomas overexpressed this protein in many more cases (65.71% of the cases). The overexpression of p53 was associated with the highest PCNA and Ki-67 LI. CONCLUSIONS: The foci of squamous metaplasia, present in colorectal adeno-carcinomas, do not seem to influence the increase of the tumours. The p53 overexpression was associated with non mucinos colorectal carcinomas and with the histological grade of colorectal adenocarcinomas. The p53 over expression tended to be more frequent in colorectal carcinomas with high proliferative activity.
AIM: To investigate the expression of PCNA, Ki-67 and p53 antibodies in colorectal carcinomas and to establish the relationship between these markers and some particular histological findings of colorectal carcinomas. MATERIAL AND METHODS: We determined immunohistochemically the expression of PCNA, Ki-67 and p53 antibodies in 41 cases of colorectal carcinomas. RESULTS: In adenocarcinomas, the tumor proliferative activity, detected with PCNA and Ki-67 antibodies, increased with the histological grade. Mucinous adenocarcinomas had a mean PCNA LI of 50% and a mean Ki-67 LI of 32%, while signet ring carcinomas had a mean PCNA LI of 70% and a mean Ki-67 LI of 45%. The proliferative activity in the foci of squamous metaplasia was lower than the proliferative activity of malignant areas in the analyzed adenocarcinomas. The p53 overexpression was detected in 24 cases (58.53%). In adenocarcinomas, the p53 positive rate increased with the dedifferentiation of these tumours. Only 16.66% of the cases of carcinomas with mucus secreting cells overexpressed p53, while adenocarcinomas overexpressed this protein in many more cases (65.71% of the cases). The overexpression of p53 was associated with the highest PCNA and Ki-67 LI. CONCLUSIONS: The foci of squamous metaplasia, present in colorectal adeno-carcinomas, do not seem to influence the increase of the tumours. The p53 overexpression was associated with non mucinos colorectal carcinomas and with the histological grade of colorectal adenocarcinomas. The p53 over expression tended to be more frequent in colorectal carcinomas with high proliferative activity.
Authors: María José García-Iglesias; Jose Luis Cuevas-Higuera; Ana Bastida-Sáenz; María Gracia de Garnica-García; Laura Polledo; Paula Perero; Jorge González-Fernández; Beatriz Fernández-Martínez; Claudia Pérez-Martínez Journal: BMC Vet Res Date: 2020-07-13 Impact factor: 2.741
Authors: Alicja Pakiet; Jarosław Kobiela; Piotr Stepnowski; Tomasz Sledzinski; Adriana Mika Journal: Lipids Health Dis Date: 2019-01-26 Impact factor: 3.876