Shengxiu Li1, Dan Goldowitz, Douglas J Swanson. 1. Department of Anatomy and Neurobiology, The University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA.
Abstract
PURPOSE: The small eye mouse mutant (Sey) is caused by a mutation of the Pax6 gene. Previous studies, in which aggregation chimeras were used, have demonstrated that Sey/Sey cells contribute poorly to the neural retina forming small clumps of cells restricted to the inner retina at embryonic day 16.5. In addition, Sey/+ cells are absent from the lens epithelium during this embryonic period and postnatally. This study was conducted to determine the fates of these Sey/Sey and Sey/+ cells with continued development in chimeric mouse eyes. METHODS: Observations were made on heterozygous and homozygous Sey cells in chimeric eyes from postnatal day (P)0 to P10. RESULTS: In Sey/Sey<-->wild-type (wt) chimeras, all Sey/Sey cells originating from retinal progenitor cells died at perinatal times. The only remaining Sey/Sey cells in the neural retina were associated with blood vessels, including vascular endothelial cells, pericytes, astrocytes, and microglia, which have extraretinal origins. In contrast, Sey/+ cells formed all retinal cell classes. As previously reported, Sey/Sey cells were absent from the lens and corneal epithelium. However, in contrast to previous reports, Sey/+ cells contributed to the lens epithelium as well as corneal tissues, and Sey/Sey cells were absent from the anterior retinal pigment epithelium. CONCLUSIONS: All evidence showed that, when Pax6 is absent at the initial stages of the development, Sey/Sey cells that contribute to the neural retina die, even when wild-type cells are available to provide normal environmental cues.
PURPOSE: The small eye mouse mutant (Sey) is caused by a mutation of the Pax6 gene. Previous studies, in which aggregation chimeras were used, have demonstrated that Sey/Sey cells contribute poorly to the neural retina forming small clumps of cells restricted to the inner retina at embryonic day 16.5. In addition, Sey/+ cells are absent from the lens epithelium during this embryonic period and postnatally. This study was conducted to determine the fates of these Sey/Sey and Sey/+ cells with continued development in chimeric mouse eyes. METHODS: Observations were made on heterozygous and homozygous Sey cells in chimeric eyes from postnatal day (P)0 to P10. RESULTS: In Sey/Sey<-->wild-type (wt) chimeras, all Sey/Sey cells originating from retinal progenitor cells died at perinatal times. The only remaining Sey/Sey cells in the neural retina were associated with blood vessels, including vascular endothelial cells, pericytes, astrocytes, and microglia, which have extraretinal origins. In contrast, Sey/+ cells formed all retinal cell classes. As previously reported, Sey/Sey cells were absent from the lens and corneal epithelium. However, in contrast to previous reports, Sey/+ cells contributed to the lens epithelium as well as corneal tissues, and Sey/Sey cells were absent from the anterior retinal pigment epithelium. CONCLUSIONS: All evidence showed that, when Pax6 is absent at the initial stages of the development, Sey/Sey cells that contribute to the neural retina die, even when wild-type cells are available to provide normal environmental cues.
Authors: Crystal L Sigulinsky; Massiell L German; Amanda M Leung; Anna M Clark; Sanghee Yun; Edward M Levine Journal: Neural Dev Date: 2015-04-27 Impact factor: 3.842
Authors: Simon A Chanas; J Martin Collinson; Thaya Ramaesh; Natalie Dorà; Dirk A Kleinjan; Robert E Hill; John D West Journal: Invest Ophthalmol Vis Sci Date: 2009-04-22 Impact factor: 4.799
Authors: Natalie J Dorà; Martine Manuel; Dirk-Jan Kleinjan; David J Price; J Martin Collinson; Robert E Hill; John D West Journal: BMC Res Notes Date: 2018-10-05
Authors: Jack W Hickmott; Uvini Gunawardane; Kimberly Jensen; Andrea J Korecki; Elizabeth M Simpson Journal: Gene Ther Date: 2018-09-26 Impact factor: 5.250