| Literature DB >> 175847 |
E Moustafa, T Skomedal, J B Osnes, I Oye.
Abstract
Adult rat heart cells were isolated by perfusion with a calcium-free phosphate buffer containing collagenase. Optimal conditions gave a high proportion of elongated cells. Isoprenaline increased cydic AMP content linearly, with ED50 (dose effective in 50% of the population) about 10(-7) M. Ca2+ made the cells spherical, and it nearly abolished cyclic AMP response as did lack of Mg2+.Entities:
Mesh：
Substances：
Year: 1976 PMID： 175847 DOI： 10.1016/0304-4165(76)90308-1
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002