| Literature DB >> 17583518 |
Franck Chopin1, Judith Wirth, Marie-France Dorbe, Laurence Lejay, Anne Krapp, Alain Gojon, Françoise Daniel-Vedele.
Abstract
Arabidopsis AtNRT2.1 protein is the best characterized high affinity nitrate transporter in higher plants. However, nothing is known about its sub-cellular localization. In this work, we used GFP imaging to follow the targeting of the AtNRT2.1 protein to the different cell membranes. A polyclonal antibody was also raised against a peptide derived from the AtNRT2.1 sequence. Comparison of wild type and mutant plant extracts showed that this antibody recognized specifically the AtNRT2.1 protein. Microsomal membranes were fractionated on sucrose gradients and immunological detections were performed on the different fractions. Altogether, our results demonstrate that the AtNRT2.1 protein is located in the plasma membrane of the root cells.Entities:
Mesh：
Substances：
Year: 2007 PMID： 17583518 DOI： 10.1016/j.plaphy.2007.04.007
Source DB: PubMed Journal: Plant Physiol Biochem ISSN： 0981-9428 Impact factor: 4.270