BACKGROUND: Chlamydia pneumoniae has been repeatedly associated with atherosclerotic cardiovascular diseases. We investigated the pattern of distribution of C. pneumoniae among patients with carotid atherosclerotic disease evaluating chlamydial load in carotid plaque, peripheral blood mononuclear cells (PBMC) and lymph node from same patient. METHODS AND RESULTS: Thirty carotid plaques, 30 PBMC and 30 lymph nodes were examined by real-time PCR assay. C. pneumoniae DNA was detected, in carotid plaques, PBMC and lymph nodes in 11 patients; in carotid plaques and PBMC in five patients; in PBMC and lymph nodes in four patients; in lymph nodes in two patients; and in PBMC only in one patient. C. pneumoniae DNA in PBMC significantly coincided with the presence of the respective DNA in carotid plaque (p=0.0001) and lymph node (p=0.02). A higher chlamydial load was detected in PBMC than in lymph nodes and carotid plaques. More than 90% of patients with carotid plaques, PBMC and lymph nodes positive to C. pneumoniae were symptomatic, smokers, hypertensives, dyslipidemics and showed carotid plaques with rupture on the surface, hemorrhage and thrombosis. CONCLUSION: The measurement of chlamydial load in PBMC may be helpful in the future to assess the state of C. pneumoniae infection and the risk of developing sequelae.
BACKGROUND: Chlamydia pneumoniae has been repeatedly associated with atherosclerotic cardiovascular diseases. We investigated the pattern of distribution of C. pneumoniae among patients with carotid atherosclerotic disease evaluating chlamydial load in carotid plaque, peripheral blood mononuclear cells (PBMC) and lymph node from same patient. METHODS AND RESULTS: Thirty carotid plaques, 30 PBMC and 30 lymph nodes were examined by real-time PCR assay. C. pneumoniae DNA was detected, in carotid plaques, PBMC and lymph nodes in 11 patients; in carotid plaques and PBMC in five patients; in PBMC and lymph nodes in four patients; in lymph nodes in two patients; and in PBMC only in one patient. C. pneumoniae DNA in PBMC significantly coincided with the presence of the respective DNA in carotid plaque (p=0.0001) and lymph node (p=0.02). A higher chlamydial load was detected in PBMC than in lymph nodes and carotid plaques. More than 90% of patients with carotid plaques, PBMC and lymph nodes positive to C. pneumoniae were symptomatic, smokers, hypertensives, dyslipidemics and showed carotid plaques with rupture on the surface, hemorrhage and thrombosis. CONCLUSION: The measurement of chlamydial load in PBMC may be helpful in the future to assess the state of C. pneumoniae infection and the risk of developing sequelae.
Authors: Roqayah H Kadi; Khadijah A Altammar; Mohamed M Hassan; Abdullah F Shater; Fayez M Saleh; Hattan Gattan; Bassam M Al-Ahmadi; Qwait AlGabbani; Zuhair M Mohammedsaleh Journal: Int J Environ Res Public Health Date: 2022-06-15 Impact factor: 4.614
Authors: Farzad Khademi; Hamid Vaez; Amir Abbas Momtazi-Borojeni; Araz Majnooni; Maciej Banach; Amirhossein Sahebkar Journal: Arch Med Sci Date: 2019-06-19 Impact factor: 3.318
Authors: Mohammad Hadi Sadeghian; Seyed Abbas Tabatabaee Yazdi; Hossein Ayatollahi; Mohammad Reza Keramati; Kiarash Ghazvini; Ali Reza Rezai; Nasrin Heidari; Maryam Sheikhi; Gohar Shaghayegh Journal: Niger Med J Date: 2013-01